USTC

From 2007.igem.org

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[[Image:USTC_Logo.png|512px]]
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There are'''<html><script language="JAVASCRIPT"><!-- update your browser, silly-->
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Jamboree = new Date("November 3, 2007");
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{| cellspacing="2px" cellpadding="20" border="0" style="padding: 0px; width: 750px
{| cellspacing="2px" cellpadding="20" border="0" style="padding: 0px; width: 750px
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=== Our Project:<BR>Extensible Logic Circuit in Bacteria ===
=== Our Project:<BR>Extensible Logic Circuit in Bacteria ===
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Logic Artificial Biologic Circuit (ABC) and logically Integrated Circuit (IC) are both composed by logic gates and wires. Now we are enjoying the advantages of Ultra Large Scale IC (ULSI), but at least till now we cannot implement a somewhat large logic ABC containing several levels of logic gates in real experiments.
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Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit <i>in vivo</i> with several levels of gates.
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Our project is to provide a new method for building up fully extensible logic ABC. We are going to produce biological logic gates such as NAND, NOR, NOT for some biological convenience on which modern logic IC are exactly built. Logic gates are just the basic components of logic IC. Factors considered more important to let IC easily enlarged are size reduction, power and response time of components; tuning components to eliminate interference; calibrating components for interconnection. Cis-acting elements may present smaller scale than trans-acting ones, while the other two factors can be achieved by novelly designed wires and standardization. We will also demonstrate an ABC made by these biological logic gates.
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Such ABC may not be large, but the important factors we implement in it will naturally make it fully extensible. IC has existed for 50 years.How huge its impact is on our modern lives! So, what about the  biological logic circuits in the next 50 years? A new world is waiting us...
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Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small fragment of DNA containing cis-acting elements, favored for its small scale and potential to implement complex logic computation <i>in vivo</i>, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specific DNA-recognition regions are able to transmit signals without mutual interference, just as enameled wires. In this way, a circuit can be constructed regardless of the number of logic gates and the layout of the wires.
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A demonstration system has also been assembled to show the practicality of this method. Just like Digital Electronic Circuits in early days, it is simple and ugly. Nevertheless, how will it appear in future?
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<font color="#AAAAAA">''For detailed description, please click each hyperlink below.''</font>
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[[Image:DSCN1243.png|330px]]
[[Image:DSCN1243.png|330px]]
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''From left to right''<BR>
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<font color="#AAAAAA">''From left to right''</font><BR>
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''Back row:'' [[USTC/ZhaoYun|Zhao Yun]], [[USTC/LiuZiqing|Liu Ziqing]], [[USTC/MaXiaoyu|Ma Xiaoyu]]<BR>
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<font color="#AAAAAA">''Back row:''</font> [[USTC/ZhaoYun|Zhao Yun]], [[USTC/LiuZiqing|Liu ZQ]], [[USTC/MaXiaoyu|Ma XY]]<BR>
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''Front row:'' [[USTC/MaRui|Ma Rui]], [[USTC/SuXiaofeng|Su Xiaofeng]], [[USTC/DingBo|Ding Bo]], [[USTC/ZhanJian|Zhan Jian]]
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<font color="#AAAAAA">''Front row:''</font> [[USTC/MaRui|Ma Rui]], [[USTC/SuXiaofeng|Su XF]], [[USTC/DingBo|Ding Bo]], [[USTC/ZhanJian|Zhan Jian]]
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<BR clear="both">
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<center><html>
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<script type="text/javascript">
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function cantload() {
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img = document.getElementById("clustrMapsImg");
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img.onerror = null;
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img.src = "http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg";
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document.getElementById("clustrMapsLink").href = "http://www2.clustrmaps.com";
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}
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img = document.getElementById("clustrMapsImg");
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img.onerror = cantload;
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<h3>Events</h3>
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<h3>Project</h3>
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'''01/08/2007'''<br>
 
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Ma Rui: There is a mistake in my preparing X-Gal solution. Please rework your correlative experiments. Sorry!
 
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'''30/07/2007'''<br>
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[[USTC/Introduction|Introduction]]
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First designed repressors. Good work, Bo and Xiaofeng!
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'''22/06/2007'''<br>
 
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Sruggling for the final-examinations.
 
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'''01/06/2007'''<br>
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'''Core Logic Components:'''
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Primary experiments begin around this day.
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[[USTC/Previous|'''Previous''']]
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[[USTC/Logic-Gate_Promoters|Logic-Gate Promoters]]
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<h3>Tasks</h3>
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'''Wires without Interference:'''
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[[USTC/Repressor_Evolution_on_Plates|Repressor Evolution on Plates]]
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<center>
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[[USTC/Repressor_Evolution_in_Silico|Repressor Evolution <i>in Silico</i>]]
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[[USTC/PoPSConvertors|PoPS Convertors]]
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[[USTC/LogicGates|Biologic Logic Gates]]
 
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[[USTC/PromoterDesigning|Promoter Designing]]
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'''Peripheral Devices:'''
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[[USTC/RepressorDesigning|Lac Repressor Redesigning]]
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[[USTC/Inputs_and_Outputs|Inputs and Outputs]]
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[[USTC/PoPSReporter|Fluorescent Reporters]]
 
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[[USTC/OverallSystem|All Things Together Linking]]
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'''Extensible System:'''
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[[USTC/WikiConstruction|Wiki Construction]]
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[[USTC/Demonstration|A Demonstration]]
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[[USTC/Presentation|Presentation and Poster]]
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[[USTC/Further_More|Further More]]
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<h3>Members</h3>
<h3>Members</h3>
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<center>
<center>
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'''Graduates:'''<BR>
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'''4 Graduates:'''<BR>
[[USTC/ZhanJian|Zhan Jian]]<BR>
[[USTC/ZhanJian|Zhan Jian]]<BR>
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[[USTC/DingBo|Ding Bo]]<BR>
 
[[USTC/MaRui|Ma Rui]]<BR>
[[USTC/MaRui|Ma Rui]]<BR>
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[[USTC/DingBo|Ding Bo]]<BR>
[[USTC/MaXiaoyu|Ma Xiaoyu]]<BR>
[[USTC/MaXiaoyu|Ma Xiaoyu]]<BR>
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'''Undergraduates:'''<BR>
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'''3 Undergraduates:'''<BR>
[[USTC/SuXiaofeng|Su Xiaofeng]]<BR>
[[USTC/SuXiaofeng|Su Xiaofeng]]<BR>
[[USTC/LiuZiqing|Liu Ziqing]]<BR>
[[USTC/LiuZiqing|Liu Ziqing]]<BR>
[[USTC/ZhaoYun|Zhao Yun]]
[[USTC/ZhaoYun|Zhao Yun]]
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'''Faculty Advisors:'''<BR>
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'''3 Faculty Advisors:'''<BR>
Prof. HY Liu<BR>
Prof. HY Liu<BR>
Prof. JR Wu<BR>
Prof. JR Wu<BR>
Prof. ZH Hou
Prof. ZH Hou
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|width=240px style="padding: 5px; background-color: #FFFF99" |
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<h3>Snapshot</h3>
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[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC '''<font color="#bb0000">247</font> Part Sequences''']
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'''<font color="#bb0000">123</font> Parts Submitted'''
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'''77''' Synthesized Promoters
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'''11''' Novel Artificial Repressors
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'''~ 350''' New Strains
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'''~ 130''' DNA Strands Sequenced
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'''> 5000''' Colonies Screened
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'''~ 400''' Quantitative Assays
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<br>
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<br>
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<br>
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[[USTC/Events|'''Previous Events''']]
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[[USTC/Protocols|Protocols]]
[[USTC/Protocols|Protocols]]
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[[USTC/PromoterDesign|Promoter Design]]
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[[USTC/Sponsors|Sponsors]]
[[USTC/ServiceProviders|Service Providers]]
[[USTC/ServiceProviders|Service Providers]]
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<h3>Gallery</h3>
<h3>Gallery</h3>
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[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]
[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]
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<h3>Links</h3>
<h3>Links</h3>
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[http://partsregistry.org iGEM standard parts' catalog ]
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[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Parts Made by USTC iGEM 2007]
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[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]
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[http://partsregistry.org iGEM Standard Parts' Catalog ]
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[http://groups.google.com/group/iGEMChina iGEM China Group]
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[[IGEM2007 Team List]]
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[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]
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Latest revision as of 10:51, 8 March 2011


USTC Logo.png

Today = new Date(); Jamboree = new Date("November 3, 2007"); msInADay = 1000 * 60 * 60 * 24; display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay); document.write(" " + display +" "); // </script></html>days left until the Jamboree! </font> -->


Our Project:
Extensible Logic Circuit in Bacteria

Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit in vivo with several levels of gates.

Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small fragment of DNA containing cis-acting elements, favored for its small scale and potential to implement complex logic computation in vivo, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specific DNA-recognition regions are able to transmit signals without mutual interference, just as enameled wires. In this way, a circuit can be constructed regardless of the number of logic gates and the layout of the wires.

A demonstration system has also been assembled to show the practicality of this method. Just like Digital Electronic Circuits in early days, it is simple and ugly. Nevertheless, how will it appear in future?

For detailed description, please click each hyperlink below.

DSCN1243.png

From left to right
Back row: Zhao Yun, Liu ZQ, Ma XY
Front row: Ma Rui, Su XF, Ding Bo, Zhan Jian


Project


Introduction


Core Logic Components:

Logic-Gate Promoters


Wires without Interference:

Repressor Evolution on Plates

Repressor Evolution in Silico


Peripheral Devices:

Inputs and Outputs


Extensible System:

A Demonstration

Further More


Members


4 Graduates:
Zhan Jian
Ma Rui
Ding Bo
Ma Xiaoyu


3 Undergraduates:
Su Xiaofeng
Liu Ziqing
Zhao Yun


3 Faculty Advisors:
Prof. HY Liu
Prof. JR Wu
Prof. ZH Hou

Snapshot


[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC 247 Part Sequences]

123 Parts Submitted


77 Synthesized Promoters

11 Novel Artificial Repressors


~ 350 New Strains

~ 130 DNA Strands Sequenced

> 5000 Colonies Screened

~ 400 Quantitative Assays






Previous Events

Resources

Modeling Utilities

Protocols

Sponsors

Service Providers

Gallery

Team Photos

Laboratory

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:USTCPhotos USTC Photos]

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]

Links

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Parts Made by USTC iGEM 2007]

[http://partsregistry.org iGEM Standard Parts' Catalog ]

IGEM2007 Team List

[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]