Bologna University/Soc medium
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< Bologna University(Difference between revisions)
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| - | ::1. To prepare 1 | + | ::1. To prepare 1 L of SOC medium dissolve in ultrapure water: |
::::: -Tryptone 4 g | ::::: -Tryptone 4 g | ||
::::: -Yeast extract 1g | ::::: -Yeast extract 1g | ||
| Line 5: | Line 5: | ||
::::: -1M KCl 0.5 ml | ::::: -1M KCl 0.5 ml | ||
::::: -5M NaOH 200 μl to adjust the pH to 6.8 | ::::: -5M NaOH 200 μl to adjust the pH to 6.8 | ||
| - | ::2 | + | ::2. After autoclaving add 2 ml each of 2M Mg-salt (1M MgSO4 and 1M MgCl) and 2M glucose. |
[[Bologna University/Mediums and buffers | Back]] | [[Bologna University/Mediums and buffers | Back]] | ||
Latest revision as of 09:23, 25 October 2007
- 1. To prepare 1 L of SOC medium dissolve in ultrapure water:
- -Tryptone 4 g
- -Yeast extract 1g
- -1M NaCl 2 ml
- -1M KCl 0.5 ml
- -5M NaOH 200 μl to adjust the pH to 6.8
- 2. After autoclaving add 2 ml each of 2M Mg-salt (1M MgSO4 and 1M MgCl) and 2M glucose.
- 1. To prepare 1 L of SOC medium dissolve in ultrapure water:
