Bologna University/Digestion reaction

< Bologna University(Difference between revisions)

Latest revision as of 09:43, 25 October 2007

(final volume 30μl)

1. Mix :

- 1μg plasmid

- 3μl Buffer 10x [ ]

- 0.3μl BSA 100x [ ]

- 3 units of restriction enzyme

- water up to 30μl

2. Incubate at 37 °C for 1hr.

3. Incubate at the inactivation temperature of the enzyme for 20min.

@@ Line 1: / Line 1: @@
+:: (final volume 30μl)
 ::1. Mix :
-::::: -1 μg of plasmid
+::::: - 1μg plasmid
-::::: -3 μl of Buffer 10X
+::::: - 3μl Buffer 10x [ ]
-::::: -0.3 μl BSA 100X
+::::: - 0.3μl BSA 100x [ ]
-::::: -3 units of restriction enzyme
+::::: - 3 units of restriction enzyme
-::::: -water up to 30 μl
+::::: - water up to 30μl
-::2. Incubate at 37 °C for 1 hr.
+::2. Incubate at 37 °C for 1hr.
-::3. Incubate at the inactivation temperature of the enzyme for 20 minutes.
+::3. Incubate at the inactivation temperature of the enzyme for 20min.
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