UCSF

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== Our Project ==
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{| cell spacing="1" style="border: 1px solid"
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|width="886px" align="center" style="font-size:24px"|
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'''Location, Location, Location: <br> Directing Biology through Synthetic Assemblies and Organelles'''
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<p style="font-size:10pt">[[UCSF/Main_Introduction|A General Introduction]]</p>
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|-
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{|style=
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|[[Image:UCSFProject1.png|left]]
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|<p style="font-size:14pt">Project 1: Protein Scaffolds as a Molecular Breadboard</p>
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Using synthetic protein scaffolds to control information flow of a kinase pathway in eukaryotic cells.<br>
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[[UCSF/Intro|Read More]]
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|-
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|[[Image:UCSFProject2.png|left]]
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|<p style="font-size:14pt">Project 2: Creating a Synthetic Organelle</p>
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Engineering phosphoinositide "barcodes" to create an intracellular membrane-bound chassis for
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Location, Location, Location: Directing Biology through Synthetic
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synthetic biology applications in eukaryotic cells. <br>
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Assemblies and Organelles
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[[UCSF/Organelle_Intro|Read More]]
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|}
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<!-- PEOPLE HEADER -->
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<h3><html><img src=http://openwetware.org/images/3/39/Icon_groups.png alt=People>
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</html><font color=black>People
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<hr/>
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<!-- END PEOPLE HEADER -->
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===Students===
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*[[Michael Chen]] (UC Berkeley)
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*[[Eric Chou]] (Lincoln High)
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*[[Jimmy Huang]] (Lincoln High)
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*[[Lauren Jann]] (Lincoln High)
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*[[Eric Meltzer]] (Palo Alto High)
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*[[Alexander Ng]] (Lincoln High)
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*[[Robert Ovadia]] (Lincoln High)
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We have taken a new and exciting approach to the iGEM challenge this year.  Because UCSF is without an undergraduate program, we chose to take on 7 exceptional students (6 high school students and 1 undergraduate) from the bay area. The students' participation was conceived with help from UCSF's SEP program — the Science and Health Education Partnership between UCSF and the San Francisco Unified School District, designed to help boost student science literacy in the city. Using iGEM as a guiding framework, we created an educational program to introduce them to Synthetic Biology and experimental science.  As part of this, the students worked as a team with a post-baccalaureate instructor and a group of graduate students and post-doctoral researchers to actively create an interesting and fun project.
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===Instructors===
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This year, we are using synthetic approaches to engineer and manipulate novel cellular microenvironments into Eukaryotic cells. It is widely appreciated that the efficiency and specificity of many cellular processes frequently depend on spatial localization, which can be achieved in different ways. For example, multiple enzymes involved in a given process frequently co-localize by binding to a common scaffold. Processes can also be localized in larger and more specialized microenvironments, such as whole organelles, through physical separation into distinct membrane compartments.
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*[[Noah Helman]]
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Co-localization and compartmentalization allow molecular components to function in concert more effectively, and can protect the process from the external environment. Conversely, if the process involves any harmful intermediates (e.g. degradation, oxidation, etc.), compartmentalization can protect the rest of the cell. This summer, our team is creating novel microenvironments in yeast through (a) protein-scaffold interactions and (b) membrane compartmentalization. In the first project, we are reengineering the pathway output of the yeast mating response through the recruitment of exogenous pathway modulators to the Ste5 scaffold via synthetic leucine zippers. Our approach uses a new combinatorial cloning method based on type IIs restriction enzymes, allowing multi-step ligations to be consolidated into one. In a second project, we are using the same signaling pathway to engineer a new organelle in yeast. We are exploiting the observation that a unique code of modified phosphoinositide lipids is usually used to confer distinct identities to membranous compartments (i.e. organelles). Therefore, our project is based on conferring a distinct phosphoinositide composition to endosomes containing the mating receptor Ste2 by recruiting mammalian phosphoinositide phosphatases (MTMRs) or kinases (PI3K) able to produce phosphoinositides that normally do not exist in yeast. Through this, we hope to arrest trafficking of Ste2-specific endosomes to the yeast vacuole, creating a new stable organelle labeled with a unique and orthogonal molecular identity code. Creation of a new organelle could have unlimited engineering potential and a wide array of applications, such as creation of a segregated compartment dedicated to the synthesis of drugs or biofuels.
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*[[Andrew Horwitz]]
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*[[Sergio Peisajovich]]
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*[[David Pincus]]
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*[[Ryan Ritterson]]
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*[[Nili Sommovilla]]
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*[[Reid Williams]]
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*[[Angela Won]]
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== More ==
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===Faculty Advisors===
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[http://openwetware.org/wiki/IGEM:UCSF/2007 Visit the UCSF Team Homepage on OpenWetWare!]
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*[http://www.ucsf.edu/limlab/ Wendell Lim]
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*[[Hana El-Samad]]
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*[http://kortemmelab.ucsf.edu/ Tanja Kortemme]
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*[http://www.voigtlab.ucsf.edu/ Chris Voigt]
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===Planning & Development===
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*[[/Weekly Meetings/]]
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*[[/Past Presentations/]]
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*[[/Papers of Interest/]]
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*[[Logo Galore...]]
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===Media===
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*[http://igem.ucsf.edu UCSF iGEM Homepage]
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*[[IGEM:UCSF/2007/Photos | Photos]]
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*[[/Videos/]]
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*[http://169.230.117.36/today/cache/feature/200708022.html in ucsf news!]
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*Who's been checking us out?
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<html> <a href="http://www3.clustrmaps.com/counter/maps.php?url=http://openwetware.org/wiki/IGEM:UCSF/2007"><img src="http://www3.clustrmaps.com/stats/maps-no_clusters/openwetware.org-wiki-IGEM-UCSF-2007-thumb.jpg" />
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</a> </html></font>
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<!-- COMMUNITY HEADER -->
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<html><h3>
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<img src="http://openwetware.org/images/b/b9/Icon_board.png" alt=Resources>
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</html>The Project</h3>
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<hr/>
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===About Us===
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*[[/Our Story/]]
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*[http://igem.ucsf.edu Our official website]
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===A Guided Tour through the Project===
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*[[/Project Description/]]
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*[[/Main Introduction/]]
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*'''Project One: Engineering Protein Complexes'''
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**[[/Intro/]]
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**[[/Results/]]
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**[[/Conclusions/]]
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*'''Project Two: The Synthetic Organelle'''
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**[[/Organelle Intro/| Intro]]
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**[[/Results_P2/| Results]]
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**[[/Conclusions_P2/| Status]]
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*[[/Overall Conclusions/| Overall Conclusions]]
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===Behind the Scenes===
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*'''Methods and Materials'''
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**'''Project One'''
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***[[/Cloning Strategy1/| Cloning Strategy]]
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***[[/YeastStrains1/| Yeast Strains]]
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***[[/Flow Cytometry1/| Flow Cytometry]]
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**'''Project Two'''
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***[[/Cloning Strategy2/| Cloning Strategy]]
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***[[/YeastStrains2/|Yeast Strains]]
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***[[/Flow Cytometry2/|Flow Cytometry]]
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***[[/Microscopy/|Microscopy]]
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*[[IGEM:UCSF/2007/Plasmids | Plasmids & Sequences]]
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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__NOTOC__

Latest revision as of 17:30, 18 April 2008

Logo6.png

Location, Location, Location:
Directing Biology through Synthetic Assemblies and Organelles

A General Introduction

UCSFProject1.png

Project 1: Protein Scaffolds as a Molecular Breadboard

Using synthetic protein scaffolds to control information flow of a kinase pathway in eukaryotic cells.
Read More

UCSFProject2.png

Project 2: Creating a Synthetic Organelle

Engineering phosphoinositide "barcodes" to create an intracellular membrane-bound chassis for

synthetic biology applications in eukaryotic cells.
Read More

People People

Students

Instructors

Faculty Advisors

  • [http://www.ucsf.edu/limlab/ Wendell Lim]
  • Hana El-Samad
  • [http://kortemmelab.ucsf.edu/ Tanja Kortemme]
  • [http://www.voigtlab.ucsf.edu/ Chris Voigt]

Planning & Development

Media

  • [http://igem.ucsf.edu UCSF iGEM Homepage]
  • Photos
  • Videos
  • [http://169.230.117.36/today/cache/feature/200708022.html in ucsf news!]
  • Who's been checking us out?


Resources The Project

About Us

  • Our Story
  • [http://igem.ucsf.edu Our official website]

A Guided Tour through the Project

Behind the Scenes
Retrieved from "http://2007.igem.org/wiki/index.php/UCSF"