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	::1. Mix :		::1. Mix :
-	::::: - 8 μl of insert DNA	+	::::: - 8μl of insert DNA
-	::::: - 4 μl of vector plasmid	+	::::: - 4μl of vector plasmid
-	::::: - 4 μl of Buffer 5X	+	::::: - 4μl of Buffer 5x [ ]
-	::::: - 1 μl of T4 Ligase	+	::::: - 1μl of T4 Ligase
-	::::: - 3 μl of mQ water	+	::::: - 3μl of ultrapure water
-	::2. Incubate at 4 °C overnigth or at 15 °C for 3 hr.	+	::2. Incubate at 4 °C overnigth or at 15 °C for 3hr.

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Latest revision as of 09:41, 25 October 2007

(final volume 20 μl)

1. Mix :

- 8μl of insert DNA

- 4μl of vector plasmid

- 4μl of Buffer 5x [ ]

- 1μl of T4 Ligase

- 3μl of ultrapure water

2. Incubate at 4 °C overnigth or at 15 °C for 3hr.

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