Paris/August 25
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== GLYCEROL STOCKS == | == GLYCEROL STOCKS == | ||
| + | We made glycerol stocks of the W121 strains transformed with [DapAp>>mRFP] construction<br> | ||
| + | |||
| + | {| | ||
| + | |- style="background: #ccccff;" | ||
| + | ! colspan="5" style="background: #ccccff;" | Strains glycerol stock | ||
| + | |- style="background: #ccccff; text-align: center;" | ||
| + | |width=5%| Number | ||
| + | |width=25%| Name | ||
| + | |width=55%| Description | ||
| + | |width=5%| Verified clone | ||
| + | |width=5%| Date | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |S45A | ||
| + | |L30A | ||
| + | |DapAp>>mRFP within W121 strain | ||
| + | | | ||
| + | |[[Paris/August 25| August 25]] | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |S45B | ||
| + | |L30B | ||
| + | |DapAp>>mRFP within W121 strain | ||
| + | | | ||
| + | |[[Paris/August 25| August 25]] | ||
| + | |} | ||
== Recombination Rate Test == | == Recombination Rate Test == | ||
| + | |||
| + | * We counted how many clones grew up on AMP-KAN : <br> | ||
| + | - FX85 L26.1 : 78%<br> | ||
| + | - FX85 L26.2 : 93%<br> | ||
| + | <br> | ||
| + | In all cases, we are up to the 50% max necessary to permit the spread of the population. As François-Xavier told us, CRE seems really a too good worker for our stuff !<br> | ||
| + | |||
| + | * ??? No clones from the dillution culture appeared on our AMP-THYMINE plates ??? We decide to try again with 10-7 and 10-5 dillution so as to check if the lack of clones would come from a too high dillution. | ||
Latest revision as of 13:25, 28 August 2007
GLYCEROL STOCKS
We made glycerol stocks of the W121 strains transformed with [DapAp>>mRFP] construction
| Strains glycerol stock | ||||
|---|---|---|---|---|
| Number | Name | Description | Verified clone | Date |
| S45A | L30A | DapAp>>mRFP within W121 strain | August 25 | |
| S45B | L30B | DapAp>>mRFP within W121 strain | August 25 | |
Recombination Rate Test
- We counted how many clones grew up on AMP-KAN :
- FX85 L26.1 : 78%
- FX85 L26.2 : 93%
In all cases, we are up to the 50% max necessary to permit the spread of the population. As François-Xavier told us, CRE seems really a too good worker for our stuff !
- ??? No clones from the dillution culture appeared on our AMP-THYMINE plates ??? We decide to try again with 10-7 and 10-5 dillution so as to check if the lack of clones would come from a too high dillution.
