Paris/August 26
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| + | [[Paris/August 25|yesterday]] -- [[Paris/August 27|tomorrow]] | ||
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== Recombination rate test == | == Recombination rate test == | ||
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| + | Still no clones were present on our plates... At least, we know now that the CRE, even without any stimulation of the pBAD promotor, is expressed too high for a minimal conservation of our sex line.<br> | ||
| + | Ariel gave us the advice to look for a new mutated version of lox site for the CRE recombinase. This would permit to reduce the efficiency of the CRE recombination. Let's see...<br> | ||
| + | There is also the way to use other recombination sites, as the Att sites or Dif or Flp... I'll take out the Att site biobricks tomorrow. | ||
Latest revision as of 13:37, 28 August 2007
Recombination rate test
Still no clones were present on our plates... At least, we know now that the CRE, even without any stimulation of the pBAD promotor, is expressed too high for a minimal conservation of our sex line.
Ariel gave us the advice to look for a new mutated version of lox site for the CRE recombinase. This would permit to reduce the efficiency of the CRE recombination. Let's see...
There is also the way to use other recombination sites, as the Att sites or Dif or Flp... I'll take out the Att site biobricks tomorrow.
