NYMU Taipei/Lab Notes/2007 8 29

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< NYMU Taipei/Lab Notes(Difference between revisions)
 
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*Plasmid extraction:
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*Plasmid extraction for [pOmpC+(RBS+EYFP)]
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**pOmpC+EYFP 1-1, 1-2, 2, 3, 4, 5
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**pOmpC+(RBS+EYFP) 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
**Extract from 1.5ml LB medium to 20ul EB buffer
**Extract from 1.5ml LB medium to 20ul EB buffer
**Stored in -20°C
**Stored in -20°C
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**Excitation: 514nm
**Excitation: 514nm
**Emition: 527nm
**Emition: 527nm
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*Liquid culture for MGC clones
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** INS (human), IGFBP3 (human), IGFBP7 (human), IDE (mouse) for insulin project
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** PUM1 (human), AKAP1 (human) for m.t. project
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----
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==Who attend?==
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* 立翔

Latest revision as of 07:19, 30 August 2007

  • Plasmid extraction for [pOmpC+(RBS+EYFP)]
    • pOmpC+(RBS+EYFP) 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
    • Extract from 1.5ml LB medium to 20ul EB buffer
    • Stored in -20°C
  • EYFP spectral characteristics:
    • Excitation: 514nm
    • Emition: 527nm
  • Liquid culture for MGC clones
    • INS (human), IGFBP3 (human), IGFBP7 (human), IDE (mouse) for insulin project
    • PUM1 (human), AKAP1 (human) for m.t. project

Who attend?

  • 立翔
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