Glasgow/Drylab full

From 2007.igem.org

< Glasgow(Difference between revisions)
 
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!align=center|[https://2007.igem.org/Glasgow https://static.igem.org/mediawiki/2007/thumb/c/cc/Uog.jpg/50px-Uog.jpg] ||  [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]]
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!align=center|[https://2007.igem.org/Glasgow https://static.igem.org/mediawiki/2007/thumb/c/cc/Uog.jpg/50px-Uog.jpg] ||  [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Wetlab|<font face=georgia color=#99CCFF size=4>Back To <br> Glasgow's <br> Wetlab Log</font>]] ||  [[Glasgow/Drylab_full|<font face=georgia color=#3366CC size=4><br> <br></font>]]
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|[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=#99CCFF size=5><b>Protocols</b></font>]
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|[https://2007.igem.org/Glasgow/DryTutorials <font face=georgia color=#99CCFF size=5><b>Tutorials</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>]
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|[https://2007.igem.org/Glasgow/DryReferences <font face=georgia color=#3366CC size=5><b>References</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=#99CCFF size=5><b>Resources</b></font>]
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|[https://2007.igem.org/Glasgow/DryResources <font face=georgia color=#99CCFF size=5><b>Resources</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Orders</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#99CCFF size=5><b>Biobricks<br>Used</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>]
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!width="50%" | [https://2007.igem.org/Glasgow/Wetlab/Week1 <font face=georgia color=#3366CC size=7><b>Week 1</b></font>] <br>  
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!width="50%" | [https://2007.igem.org/Glasgow/DryWeek1 <font face=georgia color=#3366CC size=7><b>Week 1</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week2 <font face=georgia color=#3366CC size=7><b>Week 2</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/DryWeek2 <font face=georgia color=#3366CC size=7><b>Week 2</b></font>] <br>  
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*'''''First selection of biobricks transformed.'''
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*'''''All the new things to be learned before the fun begins.'''
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*'''''Preparation of stocks.'''
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*'''''Change of plan because DmpR failed to grow. '''
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*'''''A taste of Multidisciplinary Research life and its bitter-sweetness!'''
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*'''''Researched XylR and DntR sensor systems.'''
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*'''''All those boooooring mathematical equation we were taught during our studies are actually becoming an applicable to real world?! It starts to make sense why we were thought these things after all…'''
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*'''''Designed primers for DmpR, DntR, XylR and associated promoters.'''
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|- align="center"
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week3 <font face=georgia color=#99CCFF size=7><b>Week 3</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/DryWeek3 <font face=georgia color=#99CCFF size=7><b>Week 3</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week4 <font face=georgia color=#99CCFF size=7><b>Week 4</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/DryWeek4 <font face=georgia color=#99CCFF size=7><b>Week 4</b></font>] <br>  
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*'''BioBricks tested by digestion and PCR.'''
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*'''''Introduction of stochastic unofficially separated modellers into two groups.'''
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*'''PCR with new primers associated with DntR and XylR.'''
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*'''''Trial and Error makes it perfect?'''
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* '''*m and *s cloned into TOPO vectors.'''
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*'''''New tools for the trade.'''
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*'''Primer design for 7 gene operon.'''
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*'''''BioBrick Concept and all the fuss about it.'''
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*'''DntR cloned into TOPO vector.'''
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*'''Primer design for XylR and associated genes.'''
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*'''Death gene cloned into TOPO vector.'''
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*'''Primer design for death gene.'''
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|- align="center"
|- align="center"
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week5 <font face=georgia color=#3366CC size=7><b>Week 5</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/DryWeek5 <font face=georgia color=#3366CC size=7><b>Week 5</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week6 <font face=georgia color=#3366CC size=7><b>Week 6</b></font>] <br>
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!width="50%" |[https://2007.igem.org/Glasgow/DryWeek6 <font face=georgia color=#3366CC size=7><b>Week 6</b></font>] <br>
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*'''Suspected amplification of 7 gene operon.'''
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*'''''First real input from the lab.'''
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* '''*m and *s confirmed as present in TOPO vectors.'''
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*'''''But is it not what we expected for?'''
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*'''Confirmed presence of death gene plasmid in some vectors.'''
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*'''Miller assay performed with DntR system.'''
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*'''Yeast cells.'''
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*'''Biobricks made of (*m), (*s), and DntR.'''
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*'''''From trial to final model.'''
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*'''''The Black Art of Programing'''
|- align="center"
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week7 <font face=georgia color=#99CCFF size=7><b>Week 7</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/DryWeek7 <font face=georgia color=#99CCFF size=7><b>Week 7</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week8 <font face=georgia color=#99CCFF size=7><b>Week 8</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/DryWeek8 <font face=georgia color=#99CCFF size=7><b>Week 8</b></font>] <br>  
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*'''Utilised new biobricks.'''
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*'''''Models are being created by the dozen.'''
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*'''Site directed mutagenesis of (*m) completed.'''
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*'''''A grant for multicore computing - giant leap for our modeling.'''
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*'''Site directed mutagenesis of (*s) completed.'''
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*'''''Initiation of documentation.'''
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*'''(*d→g*) successfully cloned into TOPO vector.'''
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*'''''Boooooooring.'''
|- align="center"
|- align="center"
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week9 <font face=georgia color=#3366CC size=7><b>Week 9</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/DryWeek9 <font face=georgia color=#3366CC size=7><b>Week 9</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week10 <font face=georgia color=#3366CC size=7><b>Week 10</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/DryWeek10 <font face=georgia color=#3366CC size=7><b>Week 10</b></font>] <br>  
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*'''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''
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*'''''Our enemy LaTeX.'''
 +
*'''''And other usefull stuf.'''
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*'''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
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*'''''Project declaration.'''
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*'''''The End.'''
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{|cellspacing="6px" cellpadding="16" border="0" width="100%"
{|cellspacing="6px" cellpadding="16" border="0" width="100%"
|- align=center
|- align=center
-
|[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=#99CCFF size=5><b>Protocols</b></font>]
+
|[https://2007.igem.org/Glasgow/DryTutorials <font face=georgia color=#99CCFF size=5><b>Tutorials</b></font>]
-
|[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>]
+
|[https://2007.igem.org/Glasgow/DryReferences <font face=georgia color=#3366CC size=5><b>References</b></font>]
-
|[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=#99CCFF size=5><b>Resources</b></font>]
+
|[https://2007.igem.org/Glasgow/DryResources <font face=georgia color=#99CCFF size=5><b>Resources</b></font>]
-
|[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Orders</b></font>]
+
-
|[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#99CCFF size=5><b>Biobricks<br>Used</b></font>]
+
-
|[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>]
+
|}
|}

Latest revision as of 12:05, 2 October 2007

https://static.igem.org/mediawiki/2007/thumb/c/cc/Uog.jpg/50px-Uog.jpg Back To
Glasgow's
Main Page 		Back To
Glasgow's
Wetlab Log

Tutorials References Resources


Click on a week number for more detailed lab book...


Week 1
Week 2
  • All the new things to be learned before the fun begins.
  • A taste of Multidisciplinary Research life and its bitter-sweetness!
  • All those boooooring mathematical equation we were taught during our studies are actually becoming an applicable to real world?! It starts to make sense why we were thought these things after all…
Week 3
Week 4
  • Introduction of stochastic unofficially separated modellers into two groups.
  • Trial and Error makes it perfect?
  • New tools for the trade.
  • BioBrick Concept and all the fuss about it.
Week 5
Week 6
  • First real input from the lab.
  • But is it not what we expected for?
  • From trial to final model.
  • The Black Art of Programing
Week 7
Week 8
  • Models are being created by the dozen.
  • A grant for multicore computing - giant leap for our modeling.
  • Initiation of documentation.
  • Boooooooring.
Week 9
Week 10
  • Our enemy LaTeX.
  • And other usefull stuf.
  • Project declaration.
  • The End.
Tutorials References Resources
Retrieved from "http://2007.igem.org/wiki/index.php/Glasgow/Drylab_full"