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Revision as of 08:20, 5 October 2007 (view source) Blent (Talk | contribs) (→PCR length check) ← Older edit		Latest revision as of 05:17, 9 October 2007 (view source) Blent (Talk | contribs) (→PCR length check)
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	== PCR length check ==		== PCR length check ==
-	* expected lengths of three ligations	+	* PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR
-	** (I) pCinRHSL (vector) + OmpRBS (insert):	+	* expected combined insertion lengths of three ligations
-	** (II) pOmpC (vector) + TATA_INSA (insert)	+	** (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613
-	** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)	+	*** 291 = pCinRHSL (225) + Sequence after left cutting sites (1) + OmpRBS (65)
		+	** (II) pOmpC (vector) + TATA_INSA (insert): 297 + 316 = 603
		+	*** 297 = pOmpC (108) + Sequence after left cutting sites (23) + TATA (90) + INSA (66)
		+	** <s>(III) TATB_INSB (insert) + CinR+HSL+D-term (vector): 1,829 + 316 = 2,145</s>

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Latest revision as of 05:17, 9 October 2007

PCR length check

• PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR
• expected combined insertion lengths of three ligations
  • (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613
    • 291 = pCinRHSL (225) + Sequence after left cutting sites (1) + OmpRBS (65)
  • (II) pOmpC (vector) + TATA_INSA (insert): 297 + 316 = 603
    • 297 = pOmpC (108) + Sequence after left cutting sites (23) + TATA (90) + INSA (66)
  • (III) TATB_INSB (insert) + CinR+HSL+D-term (vector): 1,829 + 316 = 2,145