Melbourne/Lab BL Notebook/PrimersI
From 2007.igem.org
< Melbourne | Lab BL Notebook(Difference between revisions)
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Number of Bases: 41 | Number of Bases: 41 | ||
Tm:70 | Tm:70 | ||
| - | + | == Oligo 15 == | |
Sequence Name: BL_FP1_s | Sequence Name: BL_FP1_s | ||
Sequence: AGCTAGGaattcgcggccgcttctagATGGTGGGACTTACGACCCTCT | Sequence: AGCTAGGaattcgcggccgcttctagATGGTGGGACTTACGACCCTCT | ||
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Tm:74 | Tm:74 | ||
*(fwd primer: prefix-SopII) | *(fwd primer: prefix-SopII) | ||
| + | |||
| + | ==Sequencing primer for Blue photosensor construct (spans stitch)== | ||
| + | |||
| + | -- Oligo 16 -- | ||
| + | Sequence Name: BL_seq_v1 | ||
| + | Sequence: ACCTCGATGTCGAGCTTGAG | ||
| + | Number of Bases: 20 | ||
| + | Tm:54 | ||
| + | |||
| + | ==PsrfA Primers== | ||
| + | |||
| + | -- Oligo 17 -- | ||
| + | Sequence Name: BL_psrfA_fwd | ||
| + | Sequence: AGCTAGGaattcgcggccgcttctagATGGTGGGACTTACGACCCTCT | ||
| + | Number of Bases: 48 | ||
| + | Tm:74 | ||
| + | |||
| + | -- Oligo 18 -- | ||
| + | Sequence Name: BL_psrfA_rev | ||
| + | Sequence: GGGATTGGTAAGCTGGTTTCAGtactagtagcggccgctgcagagctag | ||
| + | Number of Bases: 49 | ||
| + | Tm:74 | ||
Latest revision as of 06:54, 12 October 2007
Contents |
Primers used for PCR stitching of Blue photoreceptor
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-- Oligo 1 --
Sequence Name: BL_Con1_s Sequence: Cagtcggtgcggacatcgttgcttgtaagcaaagcttatac Number of Bases: 41 Tm:68
-- Oligo 2 --
Sequence Name: BL_Con1_as Sequence: GTATAAGCTTTGCTTACAAGCAACGATGTCCGCACCGACTG Number of Bases: 41 Tm:68
-- Oligo 3 --
Sequence Name: BL_Con2_s Sequence: TcggtgcggacatcgctgCTTGTAAGCAAAGCTTATACCTTTG Number of Bases: 43 Tm:69
-- Oligo 4 --
Sequence Name: BL_Con2_as Sequence: CAAAGGTATAAGCTTTGCTTACAAGCAGCGATGTCCGCACCGA Number of Bases: 43 Tm:69
-- Oligo 5 --
Sequence Name: BL_Con3_s Sequence: GGTGCGGACATCGCTGGAGGTAAGCAAAGCTTATACCTTTGAG Number of Bases: 43 Tm:70
-- Oligo 6 --
Sequence Name: BL_Con3_as Sequence: CTCAAAGGTATAAGCTTTGCTTACCTCCAGCGATGTCCGCACC Number of Bases: 43 Tm:70
-- Oligo 7 --
Sequence Name: BL_Con4_s Sequence: GCGGACATCGCTGGAGGACAGCAAAGCTTATACCTTTGAGGT Number of Bases: 42 Tm:70
-- Oligo 8 --
Sequence Name: BL_Con4_as Sequence: ACCTCAAAGGTATAAGCTTTGCTGTCCTCCAGCGATGTCCGC Number of Bases: 42 Tm:70
-- Oligo 9 --
Sequence Name: BL_Con5_s Sequence: GACATCGCTGGAGGACGCCAAAGCTTATACCTTTGAGGTTACTC Number of Bases: 44 Tm:70
-- Oligo 10 --
Sequence Name: BL_Con5_as Sequence: GAGTAACCTCAAAGGTATAAGCTTTGGCGTCCTCCAGCGATGTC Number of Bases: 44 Tm:70
-- Oligo 11 --
Sequence Name: BL_Con6_s Sequence: gacgcagagcaggcacaaaagGTGATATTTTTAGATAAGCATGAAGTT Number of Bases: 48 Tm:67
-- Oligo 12 --
Sequence Name: BL_Con6_as Sequence: AACTTCATGCTTATCTAAAAATATCACCTTTTGTGCCTGCTCTGCGTC Number of Bases: 48 Tm:67
-- Oligo 13 --
Sequence Name: BL_Con7_s Sequence: GGCAGAGGAGATCAACACGGAAGTTGGACCGGACTGGAATT Number of Bases: 41 Tm:70
-- Oligo 14 --
Sequence Name: BL_Con7_as Sequence: AATTCCAGTCCGGTCCAACTTCCGTGTTGATCTCCTCTGCC Number of Bases: 41 Tm:70
Oligo 15
Sequence Name: BL_FP1_s Sequence: AGCTAGGaattcgcggccgcttctagATGGTGGGACTTACGACCCTCT Number of Bases: 48 Tm:74 *(fwd primer: prefix-SopII)
Sequencing primer for Blue photosensor construct (spans stitch)
-- Oligo 16 --
Sequence Name: BL_seq_v1 Sequence: ACCTCGATGTCGAGCTTGAG Number of Bases: 20 Tm:54
PsrfA Primers
-- Oligo 17 --
Sequence Name: BL_psrfA_fwd Sequence: AGCTAGGaattcgcggccgcttctagATGGTGGGACTTACGACCCTCT Number of Bases: 48 Tm:74
-- Oligo 18 --
Sequence Name: BL_psrfA_rev Sequence: GGGATTGGTAAGCTGGTTTCAGtactagtagcggccgctgcagagctag Number of Bases: 49 Tm:74
