BerkiGEM2007-SamanthaConstructionFile4
From 2007.igem.org
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PCR sil02F and sil02R on colE2 colicin region (1758bp, BglII/XhoI) <br> | PCR sil02F and sil02R on colE2 colicin region (1758bp, BglII/XhoI) <br> | ||
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) <br> | Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) <br> | ||
| - | Product is pBca9145- | + | Product is pBca9145-I716209 |
---- | ---- | ||
Latest revision as of 18:23, 5 June 2007
Biobricking of ceaB without start codon
PCR sil02F and sil02R on colE2 colicin region (1758bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716209
sil02F Forward BglII biobrick of ceaB ccaaaAGATCTagcggtggcgatggacgcgg
sil02R Reverse XholI/BamHI biobrick of ceaB gctagCTCGAGttaGGATCCttacttaccccgatgaatatc
