Melbourne/Lab BL Notebook/20071005PCR2
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< Melbourne | Lab BL Notebook(Difference between revisions)
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| + | PCR reaction to attach prefix and suffix to the PsrfA promoter. | ||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; background: #f9f9f9; border: 1px #aaa solid; border-collapse: collapse;" | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; background: #f9f9f9; border: 1px #aaa solid; border-collapse: collapse;" | ||
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! PCR mix | ! PCR mix | ||
! PCR program | ! PCR program | ||
| - | |||
|- | |- | ||
|5ul 10x buffer\\ | |5ul 10x buffer\\ | ||
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| 50ul Total | | 50ul Total | ||
|} | |} | ||
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| + | => No product. Need to play around with conditions. | ||
Latest revision as of 10:51, 11 October 2007
PCR reaction to attach prefix and suffix to the PsrfA promoter.
| PCR mix | PCR program |
|---|---|
| 5ul 10x buffer\\
2.5ul 10x Enhancer 0.6ul dNTPs (25mM stock) 2.5ul MgSO4 (Supplied in PCR kit) 1.5ul Primer BL_psrfA_fwd (10uM stock) 1.5ul Primer BL_psrfA_rev (10uM stock) 1ul Template (pJS34) 0.4ul Pfx Platinum (Invitrogen) 32.9ul ddH2O | 94°C - 5'
94°C - 30" 50°C - 35" 68°C - 1' (goto step 2 x30) 68°C -10' 4°C forever |
| 50ul Total |
=> No product. Need to play around with conditions.
