Paris/Constructs

From 2007.igem.org

< Paris(Difference between revisions)
 
(27 intermediate revisions not shown)
Line 1: Line 1:
-
Voici un exemple de présentation de construction de biologie moléculaire. La case info sert à renvoyer vers les pages où les manips ont étés faites pour chaque partie, ou pour tout autre information utile à la "part".  
+
== Construct : Forward construction ==
 +
<html>
 +
<object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,29,0" width="800" height="600">
 +
<param name="movie" value="http://poupitoupou.free.fr/Construct_FW.swf">
 +
<param name="quality" value="high">
 +
<embed src="http://poupitoupou.free.fr/Construct_FW.swf" quality="high" pluginspage="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" width="500" height="750"></embed></object>
 +
</html>
-
[[Media:http://poupitoupou.free.fr/test.swf]]
+
== Construct : Backward construct ==
-
{|
+
=== Intermediate construct ===
-
|-
+
<br>
-
|
+
<html>
-
|align="center"|{{Paris_Object|
+
<object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,29,0" width="800" height="600">
-
|Nom = Lox71
+
<param name="movie" value="http://poupitoupou.free.fr/Construct_Intermediate.swf">
-
|Ref = O16+O17
+
<param name="quality" value="high">
-
|Img = Paris_O16_O17.png
+
<embed src="http://poupitoupou.free.fr/Construct_Intermediate.swf" quality="high" pluginspage="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" width="400" height="500"></embed></object>
-
|Info= [[Paris/Oligos|Sequence]]  [[Paris/July_24#Annealing_of_Lox66_and_Lox71|Annealing]]
+
</html>
-
}}
+
 
-
|
+
===  Backward construct with DapA E.Coli ===
-
|align="center"|{{Paris_Object|
+
<br>
-
|Nom = pSB1A2
+
<html>
-
|Ref = D22
+
<object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,29,0" width="800" height="600">
-
|Img = Paris_D22.png
+
<param name="movie" value="http://poupitoupou.free.fr/Construct_BW_DapE.swf">
-
|Info= [[Paris/July_23#Digestion_reactions|Digest]]
+
<param name="quality" value="high">
-
}}
+
<embed src="http://poupitoupou.free.fr/Construct_BW_DapE.swf" quality="high" pluginspage="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" width="450" height="350"></embed></object>
-
|
+
</html>
-
|-
+
 
-
|align="center"|{{Paris_Object|
+
===  Backward construct with DapA Subtilis ===
-
|Nom = BBa_E0840
+
<br>
-
|Ref = MP8
+
<html>
-
|Img = Paris_MP8.png
+
<object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,29,0" width="800" height="600">
-
|Info= [[Paris/July_18|Miniprep]]
+
<param name="movie" value="http://poupitoupou.free.fr/Construct_BW_DapS.swf">
-
}}
+
<param name="quality" value="high">
-
|align="center"|[[Image:Paris_RArrow.png]]
+
<embed src="http://poupitoupou.free.fr/Construct_BW_DapS.swf" quality="high" pluginspage="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" width="600" height="550"></embed></object>
-
|align="center"|{{Paris_Object|
+
</html>
-
|Nom = pSB1A2-Lox71
+
 
-
|Ref = L10
+
== Construct : recombinaison rate measurement ==
-
|Img = Paris_L10.png
+
<br>
-
|Info= [[Paris/July_24|Ligation]]
+
<html>
-
}}
+
<object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,29,0" width="800" height="600">
-
|align="center"|[[Image:Paris_LArrow.png]]
+
<param name="movie" value="http://poupitoupou.free.fr/Construct_MRR.swf">
-
|
+
<param name="quality" value="high">
-
|-
+
<embed src="http://poupitoupou.free.fr/Construct_MRR.swf" quality="high" pluginspage="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" width="500" height="600"></embed></object>
-
|align="center"|[[Image:Paris_DArrow.png]]
+
</html>
-
|
+
-
|align="center"|[[Image:Paris_DArrow.png]]
+
-
|
+
-
|
+
-
|-
+
-
|align="center"|{{Paris_Object|
+
-
|Nom = GFP Tri-Part
+
-
|Ref = D26
+
-
|Img = Paris_D26.png
+
-
|Info= [[Paris/July_23|Digest]]
+
-
}}
+
-
|
+
-
|align="center"|{{Paris_Object|
+
-
|Nom = pSB1A2-Lox71
+
-
|Ref = D35
+
-
|Img = Paris_D35.png
+
-
|Info= [[Paris/July_30|Digest]]
+
-
}}
+
-
|
+
-
|
+
-
|-
+
-
|align="center"|[[Image:Paris_RArrow.png]]
+
-
|align="center"|{{Paris_Object|
+
-
|Nom = lox71 - GFP Tri-Part
+
-
|Ref = L12
+
-
|Img = Paris_L12.png
+
-
|Info= [[Paris/July_30|Ligation]]
+
-
}}
+
-
|align="center"|[[Image:Paris_LArrow.png]]
+
-
|align="center"|{{Paris_Object|
+
-
|Nom = pTet>>mRFP
+
-
|Ref = MP13
+
-
|Img = Paris_MP13.png
+
-
|Info= [[Paris/August_14|Miniprep]]
+
-
}}
+
-
|
+
-
|-
+
-
|
+
-
|align="center"|[[Image:Paris_DArrow.png]]
+
-
|
+
-
|align="center"|[[Image:Paris_DArrow.png]]
+
-
|
+
-
|-
+
-
|
+
-
|align="center"|{{Paris_Object|
+
-
|Nom = lox71 - GFP Tri-Part
+
-
|Ref = D49
+
-
|Img = Paris_D49.png
+
-
|Info= [[Paris/August_15|Digest]]
+
-
}}
+
-
|
+
-
|align="center"|{{Paris_Object|
+
-
|Nom = pTet>>
+
-
|Ref = D50
+
-
|Img = Paris_D50.png
+
-
|Info= [[Paris/August_15|Digest]]
+
-
}}
+
-
|
+
-
|-
+
-
|
+
-
|align="center"| [[Image:Paris_RArrow.png]]
+
-
|align="center"|{{Paris_Object|
+
-
|Nom = pTet>>lox71 - GFP Tri-Part
+
-
|Ref = L34
+
-
|Img = Paris_L34.png
+
-
|Info= [[Paris/August_20|Ligation]] [[Paris/August_22|Miniprep]]
+
-
}}
+
-
|align="center"| [[Image:Paris_LArrow.png]]
+
-
|
+
-
|}
+

Latest revision as of 08:25, 19 October 2007

Contents

Construct : Forward construction

Construct : Backward construct

Intermediate construct


Backward construct with DapA E.Coli


Backward construct with DapA Subtilis


Construct : recombinaison rate measurement


Retrieved from "http://2007.igem.org/wiki/index.php/Paris/Constructs"