BerkiGEM2007-SamanthaConstructionFile9

From 2007.igem.org

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'''[rbs][Cre]''' <br>
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NOT DONE YET...found an ALWNI site in Cre, use BglI instead
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'''rbs library - want a weak rbs actually''' <br>
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'''3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)''' <br>
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'''[rbs][ATG]''' <br>
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'''4 Different rbs variants (1106A, 1106B, 1128A, 1090)''' <br>
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'''2 Different Cre variants (212 and 213)''' <br>
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----
----
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'''ATG Cre variant''' <br>
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Digest pBca1101-Bca1106A (BamHI/BglI, 1588+850+544bp, Large) <br>
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Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
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Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br>
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Digest pBca9145-Bca1117  (EcoRI/BglII, 3101+9bp, Large)<br>
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Product is pBca9145-I716214. <br>
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Plate on CamAmp <br>
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Product is pBca9145-I716224. <br>
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Digest pBca1101-Bca1106B (BamHI/BglI, 1588+850+544bp, Large) <br>
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Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br>
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Product is pBca9145-I716215. <br>
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Digest pBca1101-Bca1128A (BamHI/BglI, 1606+850+544bp, Large) <br>
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Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br>
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Product is pBca9145-I716216. <br>
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Digest pBca9145-Bca1090 (BamHI/BglI, 1538+553+9bp, Large) <br>
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Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br>
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Product is pBca9145-I716217. <br>
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Digest pBca1101-Bca1106A (BamHI/BglI, 1588+850+544bp, Large) <br>
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Digest pBca9145-I716213  (BglII/BglI, 2187+923bp, Small) <br>
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Product is pBca9145-I716218. <br>
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Digest pBca1101-Bca1106B (BamHI/BglI, 1588+850+544bp, Large) <br>
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'''GTG Cre variant''' <br>
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Digest pBca9145-I716213  (BglII/BglI, 2187+923bp, Small)) <br>
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Product is pBca9145-I716219. <br>
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Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
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Digest pBca9145-I716212  (EcoRI/BglII, 3101+9bp, Large)<br>
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Plate on CamAmp  <br>
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Product is pBca9145-I716225. <br>
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Digest pBca1101-Bca1128A  (BamHI/BglI, 1606+850+544bp, Large) <br>
 
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Digest pBca9145-I716213  (BglII/BglI, 2187+923bp, Small) <br>
 
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Product is pBca9145-I716220. <br>
 
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'''TTG Cre variants''' <br>
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Digest pBca9145-Bca1090  (BamHI/BglI, 1538+553+9bp, Large) <br>
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Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
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Digest pBca9145-I716213  (BglII/BglI, 2187+923bp, Small)<br>
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Digest pBca9145-I716213  (EcoRI/BglII, 3101+9bp, Large)<br>
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Product is pBca9145-I716221. <br>
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Plate on CamAmp <br>
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Product is pBca9145-I716226. <br>

Latest revision as of 22:50, 20 June 2007

[rbs][Cre]
rbs library - want a weak rbs actually
3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)


ATG Cre variant

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716224.


GTG Cre variant

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716225.


TTG Cre variants

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716226.