BerkiGEM2007-SamanthaConstructionFile9
From 2007.igem.org
(Difference between revisions)
(5 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
- | + | '''[rbs][Cre]''' <br> | |
- | + | '''rbs library - want a weak rbs actually''' <br> | |
- | + | '''3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)''' <br> | |
- | '''[rbs][ | + | |
- | ''' | + | |
- | ''' | + | |
---- | ---- | ||
+ | '''ATG Cre variant''' <br> | ||
- | Digest | + | Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> |
- | + | Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)<br> | |
- | + | Plate on CamAmp <br> | |
- | + | Product is pBca9145-I716224. <br> | |
- | + | ||
- | + | ||
- | Digest pBca9145- | + | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | Product is pBca9145- | + | |
- | + | '''GTG Cre variant''' <br> | |
- | + | ||
- | + | ||
+ | Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | ||
+ | Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)<br> | ||
+ | Plate on CamAmp <br> | ||
+ | Product is pBca9145-I716225. <br> | ||
- | |||
- | |||
- | |||
+ | '''TTG Cre variants''' <br> | ||
- | Digest | + | Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> |
- | Digest pBca9145-I716213 ( | + | Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)<br> |
- | Product is pBca9145- | + | Plate on CamAmp <br> |
+ | Product is pBca9145-I716226. <br> |
Latest revision as of 22:50, 20 June 2007
[rbs][Cre]
rbs library - want a weak rbs actually
3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)
ATG Cre variant
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716224.
GTG Cre variant
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716225.
TTG Cre variants
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716226.