Tokyo/Experimental precision and accurecy
From 2007.igem.org
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== AHL assay== | == AHL assay== | ||
| - | === | + | ===Objective: === |
To see how precisely we determine the fluorescence from GFP by removing LB media. | To see how precisely we determine the fluorescence from GFP by removing LB media. | ||
===Samples: === | ===Samples: === | ||
| - | + | placQI + GFP in pTrc99A (pos. con.) | |
<br>A4 ΔP+ GFP (neg. con.) | <br>A4 ΔP+ GFP (neg. con.) | ||
| Line 15: | Line 16: | ||
<br>(100:0), (70:30), (50:50), (30:70), (10:90), (3:97), (1:99) | <br>(100:0), (70:30), (50:50), (30:70), (10:90), (3:97), (1:99) | ||
<br>[[Tokyo/Wash |Wash]] | <br>[[Tokyo/Wash |Wash]] | ||
| - | ===Result: === | + | ===Result & conclusion: === |
| - | = | + | In proportion to the percentages of GFP expressing cells, fluorescence was increased as shown in Fig. 1. |
| + | <br>This indicates that the "[[Tokyo/Wash |wash]]" process well removes the other factors influencing the fluorescence. | ||
| + | |||
| + | == == | ||
| + | [[Tokyo/Preliminary assays |"Back to Preliminary assays"]] | ||
Latest revision as of 18:48, 26 October 2007
AHL assay
Objective:
To see how precisely we determine the fluorescence from GFP by removing LB media.
Samples:
placQI + GFP in pTrc99A (pos. con.)
A4 ΔP+ GFP (neg. con.)
Procedure:
AHL assay Standard protocol
Make 200 ul of mixtures of pos. con. and neg. con.
The mixture ratios of pos. con. and neg. con. are
(100:0), (70:30), (50:50), (30:70), (10:90), (3:97), (1:99)
Wash
Result & conclusion:
In proportion to the percentages of GFP expressing cells, fluorescence was increased as shown in Fig. 1.
This indicates that the "wash" process well removes the other factors influencing the fluorescence.
