Tokyo/sunaba2

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砂場2
 
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[[Image:tokyotech3.JPG|700px]]
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{| cellspacing="5"
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==[[Tokyo_Tech|Abstruct]]　　[[Tokyo/Model|Concept & Model]]　　[[Tokyo/Requirements |Requirements]]　　[[Tokyo/Genetic circuit|Genetic_circuit]]　　[[Tokyo/Works|Works]]　　[[Tokyo/about our team|About_our_team]]==
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<h1> ''' 1,[[Tokyo/Project |Project]]''' </h1>
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===[[Tokyo/Concept |Concept & Model]]===
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====Bacterial society that follows Pareto's principle====
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<br>[[Image:Ant society.JPG|350px]]
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<br>[[Image:TOP.JPG|350px]]
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===[[Tokyo/Requirements |Requirements]]: bistability and cell-cell communication===
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<br>[[Image:Bistability.JPG|200px]][[Image:Coexistence.JPG|250px]]
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===[[Tokyo/Genetic circuit|Genetic circuit]]:Quorum Sensing===
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<br>[[Image:Tokyo_genetic circuit_s.jpg|350px]]
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====Our construction parts are [[Tokyo/construction|Here]]!!! ====
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===[[Tokyo/simulation|Simulation]]:using parameters from our wet experiments ===
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<br>[[Image:Tokyo_simulation_s2.jpg|350px]]
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<h1>'''2,[[Tokyo/Works |Works]]'''</h1>
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<!--<h1> ''' [[Tokyo/Experiments |Experiments]]''' </h1>-->
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===[[Tokyo/Approach |Approach]]:We alternated approach of Wet and Dry.===
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<h1> '''Abstruct''' </h1>
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===2-0.Wet : [[Tokyo/Hybrid promoter|Hybrid promoter]]===
 
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====We need to develop the promoter that can receive two input,AHL & reperssor.We tryed to create and [[Tokyo/Hybrid promoter|successed]]!! ====
 
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<!--New part development:Hybrid promoterPBとして、2つの入力を受けるプロモータを開発する必要があった
 
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<br>図　概念図、配列とグラフ図
 
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[[Image:Hybrid.JPG|200px|]]
 
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===2-1.Dry : [[Tokyo/Formulation |Formulation]]===
 
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We writed the Formulation
 
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<!--リプレッサーＡ，Ｂ，シグナル分子Ｓの時間変化を式として表し、安定性解析を行った結果、Hill係数が大切であることがわかった
 
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<br>図　3つの式
 
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===2-2.Wet : [[Tokyo/Assay |Assay]]===
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====We decided the parameter of hill coefficients by the assay of AHL & IPTG====
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==1.[[Tokyo/Model|Concept & Model]]==
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<!--新しいパーツであるハイブリッドプロモータに関して、大腸菌を用いた実験から、2つの入力それぞれに対するHill係数を求めた。
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====We made a bacterial society that follows Pareto's principle as an [[Tokyo/Concepts|Ant society]] does.([[Tokyo/Model|more…]])====
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<br>図大腸菌の絵、FLAの絵、フィッティングのグラフ
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<br>[[Image:TOP.JPG|350px]]
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====1.coexistence====
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[[Image:hill AHL.JPG|200px]]|[[Image:hill IPTG.JPG|200px]]
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====2.Removal of Worker====
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====3.coexistence again====
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===2-3.Dry : [[Tokyo/Simulation |Simulation]]===
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wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。
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<!--最終日に動画と差し替え？？-->
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<br>[[Image:Tokyo_simulation_s2.jpg|350px]]
 
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===2-4.Wet : [[Tokyo/Assay |Assay]]===
 
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<!--====We determined the feasibility test of this ====-->
 
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Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。
 
<br>
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===2-5.Future works===
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==2.[[Tokyo/Requirements |Requirements]]==
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====Our requirements to construct this model are two things, bistability and coexistence by cell-cell communication.([[Tokyo/Requirements|more…]])====
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<br>[[Image:Bistability.JPG|200px]][[Image:Coexistence.JPG|250px]]
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<!--メニュー-->
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__NOTOC__
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==3.[[Tokyo/Genetic circuit|Genetic circuit]]==
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====We designed the genetic circuit to satisfy its requirements and developed [[Tokyo/new parts |new parts]] required ([[Tokyo/Genetic circuit|more…]]). ====
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<br>[[Image:Tokyo_genetic circuit_s.jpg|250px]]
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====　　　　　　　　　　Our constructed parts are [[Tokyo/construction|Here]]!!! ====
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<h1> '''3,[[Tokyo/Achievements |Achievements ]]'''</h1>
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<br>[[Tokyo/Hybrid promoter |Hybrid promoter ]]
 
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<br>[[Tokyo/Bistability analisis|Bistability analysis]]
 
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<br>[[Tokyo/Others |Others ]]
 
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<h1> '''3,[[Tokyo/About our team |About our team ]]'''</h1>
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==4.[[Tokyo/Works|Works]]==
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====The simulation using parameters obtained from wet experiments for our brand-new part indicated that the whole system becomes unstable when there are only idlers left, and then, they can differentiate into either of workers and idlers. ([[Tokyo/Works|more…]])====
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'''Students'''
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====<span style="color:#ff33cc;">Wet assay </span>====
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<br>[[Tokyo/Satoru Akama |Satoru Akama ]] | [[Tokyo/Kenichiro Iwasaki |Kenichiro Iwasaki]] | [[Tokyo/Hiroki Kawahara |Hiroki Kawahara ]] | [[Tokyo/Hajime Nakatani|Hajime Nakatani]]
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<br>[[Image:hill AHL.JPG|190px]]|[[Image:hill IPTG.JPG|190px]]
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====<span style="color:#0066ff;">Dry simulation </span>====
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<br>[[Image:Tokyo_simulation_s2.jpg|350px]]
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'''Advisors: Last year's member '''
 
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<br>[[Tokyo/Syotarou Ayukawa |Syotarou Ayukawa ]] | [[Tokyo/Akio Kobayashi|Akio Kobayashi ]]
 
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'''Instructors'''
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<br>[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]
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'''Links'''
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<h1> ''' ''' </h1>
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[[Tokyo/sunaba |sunaba ]]
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==⇒[[Tokyo/No click version|"No click version"]]==

Latest revision as of 11:27, 23 October 2007

Tokyotech3.JPG

Abstruct　　Concept & Model　　Requirements　　Genetic_circuit　　Works　　About_our_team

Abstruct

1.Concept & Model

We made a bacterial society that follows Pareto's principle as an Ant society does.(more…)


TOP.JPG

1.coexistence

2.Removal of Worker

3.coexistence again



2.Requirements

Our requirements to construct this model are two things, bistability and coexistence by cell-cell communication.(more…)


Bistability.JPGCoexistence.JPG



3.Genetic circuit

We designed the genetic circuit to satisfy its requirements and developed new parts required (more…).


Tokyo genetic circuit s.jpg

　　　　　　　　　　Our constructed parts are Here!!!

4.Works

The simulation using parameters obtained from wet experiments for our brand-new part indicated that the whole system becomes unstable when there are only idlers left, and then, they can differentiate into either of workers and idlers. (more…)

Wet assay


Hill AHL.JPG|Hill IPTG.JPG

Dry simulation


Tokyo simulation s2.jpg


⇒"No click version"

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