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Revision as of 08:53, 22 October 2007 (view source) Silvia.tamarri2 (Talk | contribs) ← Older edit		Latest revision as of 09:24, 25 October 2007 (view source) E-man (Talk | contribs)
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	:::::: -250mM KCl 1.864 g		:::::: -250mM KCl 1.864 g
	:::::: -10mM Pipes 0.302 g		:::::: -10mM Pipes 0.302 g
-	::2. Adjust the pH with KOH to 6.7.	+	::2. Adjust pH with KOH to 6.7.
	::3. Add 55mM MnCl2 (0.89 g of MnCl2x2H2O).		::3. Add 55mM MnCl2 (0.89 g of MnCl2x2H2O).
	::4. Filter with 0.22 μm filter.		::4. Filter with 0.22 μm filter.

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Latest revision as of 09:24, 25 October 2007

(always made fresh)

1. To prepare 100 ml dissolve in ultrapure water:

-15mM CaCl2 0.2205 g

-250mM KCl 1.864 g

-10mM Pipes 0.302 g

2. Adjust pH with KOH to 6.7.

3. Add 55mM MnCl2 (0.89 g of MnCl2x2H2O).

4. Filter with 0.22 μm filter.

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