Freiburg07/plasmids
From 2007.igem.org
(→New plasmids) |
Luckytaschka (Talk | contribs) (→Obtained plasmids) |
||
| (23 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | == | + | == Plasmids obtained from others== |
| + | |||
| + | |||
| + | '''Ca2+ sensor: ß-lactamase'''<br> | ||
Plasmid containing the lactamase part 1 fragment: [[Freiburg07/pAR310d-blac1-winzipA2|pAR310d-blac1-winzipA2 ]]<BR> | Plasmid containing the lactamase part 1 fragment: [[Freiburg07/pAR310d-blac1-winzipA2|pAR310d-blac1-winzipA2 ]]<BR> | ||
| Line 10: | Line 13: | ||
| + | '''Ca2+ sensor: DHFR'''<br> | ||
Plasmid containing the dhfr1 fragment: [[Freiburg07/pAR200d-GAS41reg1-dhfr1|pAR200d-GAS41reg1-dhfr1 ]]<BR> | Plasmid containing the dhfr1 fragment: [[Freiburg07/pAR200d-GAS41reg1-dhfr1|pAR200d-GAS41reg1-dhfr1 ]]<BR> | ||
| Line 16: | Line 20: | ||
| + | '''Light sensor: Phytochrom A with CFP and YFP'''<br> | ||
Plasmid containing PhyA1: [[Freiburg07/D153ah-phyA_1-406| D153ah-phyA_1-406 ]]<BR> | Plasmid containing PhyA1: [[Freiburg07/D153ah-phyA_1-406| D153ah-phyA_1-406 ]]<BR> | ||
| Line 27: | Line 32: | ||
Plasmid containing YFP: [[Freiburg07/ pAR200-YFP-Dumy| pAR200-YFP-Dumy ]]<BR> | Plasmid containing YFP: [[Freiburg07/ pAR200-YFP-Dumy| pAR200-YFP-Dumy ]]<BR> | ||
| - | |||
| - | == New plasmids == | + | '''Sequence of Calmodulin''': [[Freiburg07/Cam_yc6E_2|Cam_yc6E_2 ]]<BR> |
| + | <br> | ||
| + | |||
| + | == New plasmids (engineered by iGEM-Team-Members)== | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
Plasmid wih both lactamase fragments:[[Freiburg07/ pFR320d-blac1-winzipB1-blac2| pFR320d-blac1-winzipB1-blac2 ]]<BR> | Plasmid wih both lactamase fragments:[[Freiburg07/ pFR320d-blac1-winzipB1-blac2| pFR320d-blac1-winzipB1-blac2 ]]<BR> | ||
| - | Plasmid wih both lactamase fragments and | + | Plasmid wih both lactamase fragments and periplasmatic signal sequence:[[Freiburg07/pFR320p_b1_WinzipB1_b2| pFR320p_b1_WinzipB1_b2 ]]<BR> |
| + | |||
| + | |||
| + | '''Ca2+ sensor: ß-lactamase''' <br> | ||
| + | |||
Plasmids with calmodulin between the lactamase fragments:<BR> | Plasmids with calmodulin between the lactamase fragments:<BR> | ||
| Line 44: | Line 50: | ||
2 amino acids for linker: [[Freiburg07/pFR320p_b1_2calmo2_b2| pFR320p_b1_2calmo2_b2]]<BR> | 2 amino acids for linker: [[Freiburg07/pFR320p_b1_2calmo2_b2| pFR320p_b1_2calmo2_b2]]<BR> | ||
4 amino acids for linker: [[Freiburg07/pFR320p_b1_4calmo4_b2| pFR320p_b1_4calmo4_b2]]<BR> | 4 amino acids for linker: [[Freiburg07/pFR320p_b1_4calmo4_b2| pFR320p_b1_4calmo4_b2]]<BR> | ||
| - | 6 amino acids for linker: [[Freiburg07/| ]]<BR> | + | 6 amino acids for linker: [[Freiburg07/pFR320p_b1_6calmo6_b2| pFR320p_b1_6calmo6_b2]]<BR> |
| + | |||
| + | |||
| + | '''Ca2+ sensor: DHFR'''<br> | ||
| + | |||
| + | Plasmids with calmodulin between the dhfr fragments:<BR> | ||
| + | |||
| + | 2 amino acids for linker: [[Freiburg07/dFR320d-dhfr1-2calmo2-dhfr2A|dFR320d-dhfr1-2calmo2-dhfr2A ]]<BR> | ||
| + | 4 amino acids for linker: [[Freiburg07/dFR320d-dhfr1-4calmo4-dhfr2A|dFR320d-dhfr1-4calmo4-dhfr2A ]]<BR> | ||
| + | 6 amino acids for linker: [[Freiburg07/dFR320d-dhfr1-6calmo6-dhfr2A|dFR320d-dhfr1-6calmo6-dhfr2A ]]<BR> | ||
| + | |||
| + | |||
| + | '''Light sensor: Phytochrom A with CFP and YFP'''<br> | ||
| + | |||
| + | |||
| + | [[Freiburg07/pAR200-Fhy1Big-YFP| pAR200-Fhy1Big-YFP]]<BR> | ||
| + | [[Freiburg07/pAR200-Fhy1Small-YFP|pAR200-Fhy1Small-YFP ]]<BR> | ||
| + | [[Freiburg07/pAR200-PhyA-His-CFP|pAR200-PhyA-His-CFP ]]<BR> | ||
| + | [[Freiburg07/pAR200-YFP-Fhy1Big|pAR200-YFP-Fhy1Big ]]<BR> | ||
| + | [[Freiburg07/pAR200-YFP-Fhy1Small|pAR200-YFP-Fhy1Small ]]<BR> | ||
| + | <br> | ||
| + | |||
| + | [https://2007.igem.org/Freiburg BACK] | ||
Latest revision as of 15:51, 24 October 2007
Plasmids obtained from others
Ca2+ sensor: ß-lactamase
Plasmid containing the lactamase part 1 fragment: pAR310d-blac1-winzipA2
Plasmid containing the lactamase part 1 fragment with periplasmatic signal sequence: pAR310dp-blac1-winzipA2
Plasmid containing the lactamase part 2 fragment: pAR300d-winzipB1-blac2
Plasmid containing the lactamase part 2 fragment with periplasmatic signal sequence: pAR300dp-winzipB1-blac2
Ca2+ sensor: DHFR
Plasmid containing the dhfr1 fragment: pAR200d-GAS41reg1-dhfr1
Plasmid containing the dhfr2 fragment: pAR300d-AF10winDelV41D-dhfr2A
Light sensor: Phytochrom A with CFP and YFP
Plasmid containing PhyA1: D153ah-phyA_1-406
Plasmid containing FHY1: pGADT7ah-FHY1
Plasmid containing CFP: pAR200-cJunCFP
Plasmid containing YFP: pAR200-cFos-YFP
Plasmid containing YFP: pAR200-YFP-Dumy
Sequence of Calmodulin: Cam_yc6E_2
New plasmids (engineered by iGEM-Team-Members)
Plasmid wih both lactamase fragments: pFR320d-blac1-winzipB1-blac2
Plasmid wih both lactamase fragments and periplasmatic signal sequence: pFR320p_b1_WinzipB1_b2
Ca2+ sensor: ß-lactamase
Plasmids with calmodulin between the lactamase fragments:
2 amino acids for linker: pFR320p_b1_2calmo2_b2
4 amino acids for linker: pFR320p_b1_4calmo4_b2
6 amino acids for linker: pFR320p_b1_6calmo6_b2
Ca2+ sensor: DHFR
Plasmids with calmodulin between the dhfr fragments:
2 amino acids for linker: dFR320d-dhfr1-2calmo2-dhfr2A
4 amino acids for linker: dFR320d-dhfr1-4calmo4-dhfr2A
6 amino acids for linker: dFR320d-dhfr1-6calmo6-dhfr2A
Light sensor: Phytochrom A with CFP and YFP
pAR200-Fhy1Big-YFP
pAR200-Fhy1Small-YFP
pAR200-PhyA-His-CFP
pAR200-YFP-Fhy1Big
pAR200-YFP-Fhy1Small
