Freiburg07/Dephosphorylation
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total volume: 50 µl<BR> | total volume: 50 µl<BR> | ||
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43 µl plasmid<BR> | 43 µl plasmid<BR> | ||
1 µl SAP (shrink alkaline phosphatase)<BR> | 1 µl SAP (shrink alkaline phosphatase)<BR> | ||
Latest revision as of 15:20, 24 October 2007
Dephosphorylation
total volume: 50 µl
43 µl plasmid
1 µl SAP (shrink alkaline phosphatase)
5 µl SAP buffer (1:10)
Incubate for 30 minutes at 37°C.
Add one more 1 µl SAP.
Incubate for 20 minutes at 70°C (heat shock).
