Glasgow/Wetlab/Orders

From 2007.igem.org

< Glasgow | Wetlab(Difference between revisions)
(→[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow's Parts Page])
(→[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow <font face=georgia color=#3366CC size=4>Glasgow Parts Page</font>])
 
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== Glasgow's Parts Page ==
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== [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow <font face=georgia color=#3366CC size=4>Glasgow Parts Page</font>]==
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Visit the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow Parts Page] to see our constructs.
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Visit our Parts Page to see the parts on which we are working.<br>
 
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[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow's Parts Page]
 
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== Designed primers ==
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== <font face=georgia color=#3366CC size=4>Primers</font> ==
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== [[User:EmmaTravis|Emma]]'s primers ==
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== <font face=georgia color=#3366CC size=4>[[User:EmmaTravis|Emma]]'s Primers</font> ==
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!Name
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!align=center|[[Image:Uog.jpg]] ||  ||  [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Wetlab|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Wetlab Log</font>]]
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!align=center|[[Image:Uog.jpg]] ||  ||  [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Wetlab|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Wetlab Log</font>]] || [[Glasgow/Plan|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Project Page</font>]]
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Latest revision as of 11:28, 26 October 2007

Uog.jpg Back To
Glasgow's
Main Page 		Back To
Glasgow's
Wetlab Log		 Back To
Glasgow's
Project Page

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow Parts Page]

Visit the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow Parts Page] to see our constructs.

Primers

Name Sequence Tm (°C)
DmpR_SDM_PstI_1_for 5´ CTGGAGCAACAGCATGCGCTGTTC 3´ 65.6
DmpR_SDM_PstI_1_rev 5´ GCTCCAGTTTTCAGCGATGGCC 3´ 64.8
DmpR_SDM_PstI_2_for 5´ CTGGAGGAAGTGCTCAACCAGCAG 3´ 66.3
DmpR_SDM_PstI_2_rev 5´ CCTCCAGCGCTTCCACCAG 3´ 66.2
DmpR_Start_Prefix_1_for 5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´ 60.2
DmpR_End_Suffix_1_rev 5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´ 59.2
PhzM_SDM_PstI_1_for 5´ CCTGGAGGATGGCCTTGTTCAATTCGCC 3´ 69.9
PhzM_SDM_PstI_1_rev 5´ CCTCCAGGCCGAGCCCAG 3´ 70.6
PhzS_SDM_PstI_1_for 5´ CTGGAGTTCGCCGCGATGG 3´ 65.7
PhzS_SDM_PstI_1_rev 5´ ACTCCAGATGATCCTGCTCGCC 3´ 65.3
PhzS_SDM_PstI_2_for 5´ GTACTGGAGGATCAACTGGTTGCG 3´ 63.6
PhzS_SDM_PstI_2_rev 5´ GTACTGGAGGATCAACTGGTTGCG 3´ 63.9
PhzC_SDM_PstI_1_for 5´ CTGGAGCGCTTCGCAGCG 3´ 65.1
PhzC_SDM_PstI_1_rev 5´ CCTCCAGCAACCCGAATGG 3´ 64.2
PhzF_SDM_PstI_1_for 5´ GCACGCCACCACCTTGCC 3´ 66.9
PhzF_SDM_PstI_1_rev 5´ GGCGTGCAGCATGCAGCAGC 3´ 66.9
PhzB_SDM_EcoRI_1_for 5´ GAACTCGCGGTTGCGCTTGATCTTG 3´ 65.4
PhzB_SDM_EcoRI_1_rev 5´ GAGTTCATGAACGTCTTCCAGCAATTGCG 3´ 64.5
PhzE_SDM_EcoRI_1_for 5´ GAACTCCATGACCTCGGC 3´ 60.3
PhzE_SDM_EcoRI_1_rev 5´ GAGTTCCTCGACAACCGC 3´ 60.3
PhzE_SDM_PstI_1_for 5´ CTGGAGGGCGCGCGCAGC 3´ 71.2
PhzE_SDM_PstI_1_rev 5´ GCCCTCCAGGACCGCATCGAGATC 3´ 70.9
XylR_SDM_PstI_1_for 5´ GAGCTCCAGACCCAGGTTG 3´ 63.3
XylR_SDM_PstI_1_rev 5´ GTCTGGAGCTCGTAGCGCTC 3´ 63.3
XylR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´ 57.7
XylR_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´ 59.8
Pu_Prefix_1(After XylR) 5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´ 65.0
Pu_Suffix_1(After XylR) 5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´ 65.1
Pu_Prefix_2(Emma´s sequence) 5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´ 66.0
Pu_Suffix_2(Emma´s sequence) 5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´ 66.4
DntR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´ 61.2
DntR_Suffix_1 5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´ 55.2
DntR_Suffix_2 5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´ 55.3
Pr_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´ 58.8
Pr_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´ 57.3
p1010_for 5´ GCTGTGTATAAGGGAGCCTGA 3´ 59.4
p1010_rev 5´ TGACAGTTGACAGCGACAGC 3´ 61.3
Bbp_XylR_for_2 5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ 55.2
Bbs_XylR_rev_2 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ 61.8
Bbp_Pr_for_2 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ 60.8
Bbs_Pr_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ 61.3
Bbp_Pu_for_2 5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ 64.3
Bbs_Pu_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ 69.9
Bbp_PhzD_for 5´ GAATTCGCGGCCGCTTCTAGCACCACCGACGAGGTG 3´ 61.0
Bbs_PhzD_rev 5´ CTGCAGCGGCCGCTACTAGTATCATTCCAGCACCTCGTCG 3´ 61.4
Bbp_PhzA_for 5´ GAATTCGCGGCCGCTTCTAGATGAACGGTCAGCGGTACAGG 3´ 63.1
Bbs_PhzG_rev 5´ CTGCAGCGGCCGCTACTAGTAGTTGCAGGTAGCGGTGCTTC 3´ 61.9

Emma's Primers

Name Sequence Tm (°C)
* PhzUp 5´ TAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 65.6
* PhzLow 5´ CACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 64.8
*Bbp_PhzUp 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 63.6
*Bbs_PhzLow 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 64.2
Bbp_Gene 5´ GAATTCGCGGCCGCTTCTAG 3´ 65.7
Bbs 5´ CTGCAGCGGCCGCTACTAGTA 3´ 65.3
*Methyl_1 5´ TTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ 66.3
*Methyl_2 5´ TTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ 66.2
*Bbp_Methyl_1 5´ gaattcgcggccgcttctagaTTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ 63.9
*Bbs_Methyl_2 5´ ctgcagcggccgctactagtaTTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ 66.9
PhzM_for_1 5´ atgaataattcgaatcttgctgct 3´ 65.4
PhzM_rev_1 5´ tcaggccctggcagcgac 3´ 60.3
Bbp_PhzM_for_1 5´ gaattcgcggccgcttctagatgaataattcgaatcttgctgct 3´ 64.5
Bbs_PhzM_rev_1 5´ ctgcagcggccgctactagtatcaggccctggcagcgac 3´ 60.3
*Oxy_1 5´TCGAACACTCTAGAAAAGGAAGCACC 3´ 60.2
*Oxy_2 5´ TTTTTTGCATGCTAGCGTGGCCGTTCC 3´ 59.2
*Bbp_Oxy_1 5´ gaattcgcggccgcttctagaTCGAACACTCTAGAAAAGGAAGCACC 3´ 65.1
*Bbs_Oxy_2 5´ ctgcagcggccgctactagtaTTTTTTGCATGCTAGCGTGGCCGTTCC 3´ 66.9
PhzS_for_1 5´ atgagcgaacccatcgatat 3´ 71.2
PhzS_rev_1 5´ ctagcgtggccgttccacctg 3´ 60.2
Bbp_PhzS_for_1 5´ gaattcgcggccgcttctagatgagcgaacccatcgatat 3´ 70.9
Bbs_PhzS_rev_1 5´ ctgcagcggccgctactagtactagcgtggccgttccacctg 3´ 63.3
PhzH_up 5´ CGCACGGATCCTTTCAGAATGTTC 3´ 69.9
PhzH_low 5´ GCCACGCCAAGCTTCACGCTCA 3´ 70.6
Bbp_PhzUp 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ 63.3
Bbs_PhzLow 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ 57.7
Uog.jpg Back To
Glasgow's
Main Page 		Back To
Glasgow's
Wetlab Log 		Back To
Glasgow's
Project Page
Retrieved from "http://2007.igem.org/wiki/index.php/Glasgow/Wetlab/Orders"