Cambridge/New main page

From 2007.igem.org

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[[Image:Cambridge 2007 logo.png|left]]
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<span style="font-size: 240%; font-family: Trebuchet MS, Arial, Helvetica, sans-serif">'''University of Cambridge'''</span><br/><br/>
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<span style="font-size: 300%; font-family: Trebuchet MS, Arial, Helvetica, sans-serif">'''BOL: Bacteria OnLine'''</span><br/><br/>
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<span style="font-size: 160%; color: #333333">iGEM 2007 wiki</span><br/>
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<span style="font-size: 200%; color: #333333">University of Cambridge iGEM 2007</span><br/>
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<h2 style="margin:0; background:#003366; font-size:140%; font-weight:bold; border:1px solid #002447; text-align:left; padding:0.3em 0.4em; color: white;">Welcome to the University of Cambridge's iGEM 2007 wiki!</h2>
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<h2 style="margin:0; background:#003366; font-size:140%; font-weight:bold; border:1px solid #002447; text-align:left; padding:0.3em 0.4em; color: white;">Introduction</h2>
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These are the projects we are undertaking this year:
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In order to engineer interesting and useful functions in biology, a robust and extensive range of inter- and intra-cellular signalling pathways must be available. By analogy with the Internet, such a system must allow communication between cells of different heritage and structure (different “operating systems”). In the course of this project we attempted to implement candidates for both types of signalling mechanism, and additionally made progress towards adding a new Gram-positive platform for synthetic biology to the Registry.
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<span style="font-weight:bold; font-size:120%;">Standardising the Agr signalling system</span>
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<span style="font-weight:bold; font-size:120%;">PoPS amplifier</span>
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The Accessory Gene Regulator (Agr) system in ''S. aureus'' is an oligopeptide-based quorum sensing mechanism. We aim to convert it into a reusable interbacterial signalling system for communication amongst and between both Gram-positive species (''B. subtilis'') and Gram-negative bacteria (''E. coli''), based on controllable secretion and detection of the signalling peptide AIP.
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We built a standard amplifier which can be used in any synthetic transcriptional system, taking in a standard PoPS input and giving a PoPS output of a known amplification factor. In addition, the activator-promoter pairs tested have characterised crosstalk giving the potential for a controlled multifaceted response.
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For more information, see [[Signalling project description]].
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[[Cambridge/Amplifier project|More information on the amplifier project...]]
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<span style="font-weight:bold; font-size:120%;">''Bacillus subtilis'' SynBio Chassis</span>
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<span style="font-weight:bold; font-size:120%;">Peptide signalling</span>
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Most synthetic biology so far has involved Gram-negative bacteria, which are more widely used in molecular biology research; however, the biotechnology industry relies on Gram-positive bacteria. We are trying to make a "chassis" out of ''Bacillus subtilis'', a common Gram-positive bacterium, into which genetic engineers can add BioBrick parts and control circuits.
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The Accessory Gene Regulator (Agr) system in ''S. aureus'' is an oligopeptide-based quorum sensing mechanism. We worked on converting it into a reusable interbacterial signalling system for communication amongst and between both Gram-positive species (''B. subtilis'') and Gram-negative bacteria (''E. coli''), based on controllable secretion and detection of the signalling peptide AIP.
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For more information, see [[Bacillus subtilis SynBio chassis]]
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[[Cambridge/Signalling project|More information on the signalling project...]]
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<span style="font-weight:bold; font-size:120%;">PoPS amplifier</span>
 
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We aim to build a standard amplifier which can be used in any synthetic (BioBricked) system, which would take in a standard PoPS input and give a PoPS output of a known amplification factor.
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<span style="font-weight:bold; font-size:120%;">Gram-positive chassis</span>
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For more details, have a look at the [[amplifier project]].
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Most synthetic biology so far has involved Gram-negative bacteria, which are more widely used in molecular biology research; however, the biotechnology industry relies on Gram-positive bacteria. We worked on constructing a "chassis" out of ''Bacillus subtilis'', a common Gram-positive bacterium, into which genetic engineers can add BioBrick parts and control circuits.
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[[Cambridge/Gram-positive chassis project|More information on the Gram-positive chassis...]]
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Some links to explain synthetic biology and the iGEM competition:
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*[http://www.ccbi.cam.ac.uk/iGEM2007/index.php/Main_Page Go to the Cambridge iGEM 2007 team's official wiki!] (with more information about the team and how we came up with this idea)
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* [http://en.wikipedia.org/wiki/Synthetic_biology What is synthetic biology?]
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* [http://www.nature.com/nature/comics/syntheticbiologycomic/index.html Comic of SynBio published in Nature]
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*[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Cambridge BioBricks we have contributed to the Registry]
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* [http://www.igem2006.com/story.htm The iGEM story]
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* [https://2007.igem.org/IGEM2007_Team_List Teams taking part in iGEM 2007]
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<h2 style="margin:0; background:#99ccff; font-size:140%; font-weight:bold; border:1px solid #6b8fb3; text-align:left; padding:0.3em 0.4em;">Internal links</h2>
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<span style="font-weight:bold; font-size:120%;">Groundwork</span>
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* [[Brainstorming sessions]]
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* [[Logo ideas]]
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* [[Roles of team members]]
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* [[Notes]]
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<span style="font-weight:bold; font-size:120%;">The project!</span>
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* Peptide signalling: [[Signalling project description|description]], [[Detailed_background_information_-_signalling|technical notes]]
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* ''B. subtilis'' chassis: [[Bacillus subtilis SynBio chassis|description]], [[Bacillus subtilis Technical Notes|technical notes]]
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* Amplifier project: [[Amplifier project|description]], [[Amplifier project description|technical notes]]
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* [[Experimental protocols]]
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* [[Our biobricks]]
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* [[Talks we have given]]
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* [[Useful software]]
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<span style="font-weight:bold; font-size:120%;">Others</span>
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* [[Endgame]]
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* Boston, here we come: [[Trip to Boston|the trip]], [[Flights|preferred flight times]]
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* [[Social events]]
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* [[Brochure, mug and T-shirt design]]
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* [[Help:Contents|Wiki help page]]
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* [[Friday afternoons]]
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<h2 style="margin:0; background:#99ccff; font-size:140%; font-weight:bold; border:1px solid #6b8fb3; text-align:left; padding:0.3em 0.4em;">External links</h2>
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<h2 style="margin:0; background:#99ccff; font-size:140%; font-weight:bold; border:1px solid #6b8fb3; text-align:left; padding:0.3em 0.4em;">Meet the team!</h2>
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* [https://2007.igem.org/Cambridge Our team page on the official iGEM2007 wiki]
 
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* [http://igem2007.collectivex.com/main/summary Our CollectiveX discussion site]
 
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* [http://partsregistry.org The Registry of Standard Biological Parts]
 
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* [https://2007.igem.org Official iGEM 2007 homepage]
 
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* [http://www.igem2006.com Offical iGEM 2006 website]
 
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* Past Cambridge iGEM wikis: [http://www.ccbi.cam.ac.uk/iGEM2005 2005], [http://www.ccbi.cam.ac.uk/iGEM2006 2006]
 
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[[Image:Cambridge Group photo.jpg|center|500px|The Cambridge iGEM 2007 team!]]
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''Top row, from left:'' James Brown (PhD student mentor), Jim Haseloff (faculty supervisor), Gos Micklem (faculty supervisor)
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<h2 style="margin:0; background:#99ccff; font-size:140%; font-weight:bold; border:1px solid #6b8fb3; text-align:left; padding:0.3em 0.4em;">Announcements</h2>
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''Second row, from left:'' Yi Jin Liew, John Crowe (faculty), Lovelace Soirez, Stephanie May, Yue Miao
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* Stephanie has been looking into taxis to the airport, prices can be found on [[Taxis]] or just the cheapest at [[Trip to Boston|the trip]].
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''Third row, from left:'' Zhizhen Zhao (Jane), Stefan Milde, Dmitry Malyshev, Xinxuan Soh (Sheila)  
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* Work in progress: documentation of the [[signalling project description|signalling project]], [[Bacillus subtilis SynBio chassis]] and [[amplifier project description|amplifier project description]], project description on [https://2007.igem.org/Cambridge our page on the official iGEM wiki], (continual) redesigning of wiki layout
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* Please direct any suggestions you have about the layout to the wiki people!
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* Record [[experimental protocols]] when you use them!
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* Have you done your [[visa application]]?
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<h2 style="margin:0; background:#99ccff; font-size:140%; font-weight:bold; border:1px solid #6b8fb3; text-align:left; padding:0.3em 0.4em;">Contact us!</h2>
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''Front row, from left:'' Narin Hengrung, Yi Han, David Wyatt
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[[List of team members|Meet the brains behind the project!]]
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''Not in photo:'' Jim Ajioka (faculty supervisor), Lorenz Wernisch (faculty), Tony Southall (PhD student)
The mailing list for the whole Cambridge iGEM2007 team is '''igem2007 [at] ccbi.cam.ac.uk'''
The mailing list for the whole Cambridge iGEM2007 team is '''igem2007 [at] ccbi.cam.ac.uk'''
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<h2 style="margin:0; background:#003366; font-size:140%; font-weight:bold; border:1px solid #002447; text-align:left; padding:0.3em 0.4em; color: white;">Sponsors</h2>
<h2 style="margin:0; background:#003366; font-size:140%; font-weight:bold; border:1px solid #002447; text-align:left; padding:0.3em 0.4em; color: white;">Sponsors</h2>
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<span style="font-weight:bold; font-size:120%;">Major sponsors</span>
<span style="font-weight:bold; font-size:120%;">Major sponsors</span>
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:<span class="plainlinks">[http://www.cam.ac.uk http://www.ccbi.cam.ac.uk/iGEM2007/images/a/ad/Cambridge_coat_of_arms.png]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[http://www.dna20.com http://www.ccbi.cam.ac.uk/iGEM2007/images/c/c3/DNA2_0_logo.png]</span>
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:<span class="plainlinks">[http://www.cam.ac.uk https://static.igem.org/mediawiki/2007/9/95/Cambridge_UofC_coat_of_arms.png]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[http://www.dna20.com https://static.igem.org/mediawiki/2007/1/16/Cambridge_DNA2_0_logo.png]</span>
<span style="font-weight:bold; font-size:120%;">Other sponsors</span>
<span style="font-weight:bold; font-size:120%;">Other sponsors</span>
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* The Isaac Newton Trust funded UROP scheme
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* The Isaac Newton Trust-funded UROP scheme
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* EU synbiocomm program
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* EU SynBioComm programme
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Latest revision as of 21:42, 25 October 2007

Cambridge 2007 logo.png

BOL: Bacteria OnLine

University of Cambridge iGEM 2007

Introduction

In order to engineer interesting and useful functions in biology, a robust and extensive range of inter- and intra-cellular signalling pathways must be available. By analogy with the Internet, such a system must allow communication between cells of different heritage and structure (different “operating systems”). In the course of this project we attempted to implement candidates for both types of signalling mechanism, and additionally made progress towards adding a new Gram-positive platform for synthetic biology to the Registry.

PoPS amplifier

We built a standard amplifier which can be used in any synthetic transcriptional system, taking in a standard PoPS input and giving a PoPS output of a known amplification factor. In addition, the activator-promoter pairs tested have characterised crosstalk giving the potential for a controlled multifaceted response.

More information on the amplifier project...

Peptide signalling

The Accessory Gene Regulator (Agr) system in S. aureus is an oligopeptide-based quorum sensing mechanism. We worked on converting it into a reusable interbacterial signalling system for communication amongst and between both Gram-positive species (B. subtilis) and Gram-negative bacteria (E. coli), based on controllable secretion and detection of the signalling peptide AIP.

More information on the signalling project...

Gram-positive chassis

Most synthetic biology so far has involved Gram-negative bacteria, which are more widely used in molecular biology research; however, the biotechnology industry relies on Gram-positive bacteria. We worked on constructing a "chassis" out of Bacillus subtilis, a common Gram-positive bacterium, into which genetic engineers can add BioBrick parts and control circuits.

More information on the Gram-positive chassis...

  • [http://www.ccbi.cam.ac.uk/iGEM2007/index.php/Main_Page Go to the Cambridge iGEM 2007 team's official wiki!] (with more information about the team and how we came up with this idea)
  • [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Cambridge BioBricks we have contributed to the Registry]

Meet the team!


The Cambridge iGEM 2007 team!

Top row, from left: James Brown (PhD student mentor), Jim Haseloff (faculty supervisor), Gos Micklem (faculty supervisor)

Second row, from left: Yi Jin Liew, John Crowe (faculty), Lovelace Soirez, Stephanie May, Yue Miao

Third row, from left: Zhizhen Zhao (Jane), Stefan Milde, Dmitry Malyshev, Xinxuan Soh (Sheila)

Front row, from left: Narin Hengrung, Yi Han, David Wyatt

Not in photo: Jim Ajioka (faculty supervisor), Lorenz Wernisch (faculty), Tony Southall (PhD student)

The mailing list for the whole Cambridge iGEM2007 team is igem2007 [at] ccbi.cam.ac.uk

Sponsors

We are extremely grateful to the following organisations for their support of our project:

Major sponsors

[http://www.cam.ac.uk https://static.igem.org/mediawiki/2007/9/95/Cambridge_UofC_coat_of_arms.png]     [http://www.dna20.com https://static.igem.org/mediawiki/2007/1/16/Cambridge_DNA2_0_logo.png]

Other sponsors

  • The Isaac Newton Trust-funded UROP scheme
  • EU SynBioComm programme


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