BerkiGEM2007-SamanthaConstructionFile9
From 2007.igem.org
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'''[rbs][Cre]''' <br> | '''[rbs][Cre]''' <br> | ||
'''rbs library - want a weak rbs actually''' <br> | '''rbs library - want a weak rbs actually''' <br> | ||
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| - | '''ATG Cre | + | '''ATG Cre variant''' <br> |
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | ||
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)<br> | Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)<br> | ||
| - | Plate on CamAmp | + | Plate on CamAmp <br> |
Product is pBca9145-I716224. <br> | Product is pBca9145-I716224. <br> | ||
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| - | '''GTG Cre | + | '''GTG Cre variant''' <br> |
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | ||
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)<br> | Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)<br> | ||
| - | Plate on CamAmp | + | Plate on CamAmp <br> |
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Product is pBca9145-I716225. <br> | Product is pBca9145-I716225. <br> | ||
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Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small) <br> | ||
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)<br> | Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)<br> | ||
| - | Plate on CamAmp | + | Plate on CamAmp <br> |
| - | + | Product is pBca9145-I716226. <br> | |
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| - | Product is pBca9145- | + | |
Latest revision as of 22:50, 20 June 2007
[rbs][Cre]
rbs library - want a weak rbs actually
3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)
ATG Cre variant
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716224.
GTG Cre variant
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716225.
TTG Cre variants
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716226.
