Chiba/Communication

From 2007.igem.org

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[[Image:chiba_logo.png|center]]
[[Image:chiba_logo.png|center]]
__NOTOC__
__NOTOC__
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{| style="border:0;width:100%;font-family:'Trebuchet MS'" cellpadding="20px" cellspacing="0"
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[[Chiba|Introduction]] | [[Chiba/Project_Design|Project Design]] ( [[Chiba/Engeneering_Flagella|1.Sticky Hands]] | [[Chiba/Communication|2.Communication]] | [[Chiba/Quorum_Sensing|3.Size Control]] ) |  [[Chiba/Making Marimo|Making Marimos]] |  [[Chiba/Goal|Our Goal]] || [[Chiba/Team_Members|Team Members]] | [[Chiba/Members_Only|メンバ連絡簿]]
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[[Chiba|Home]]<br>
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<span style="font-size:120%;font-weight:bold;">[[Chiba/Introduction|Introduction]] | [[Chiba/Project_Design|Project Design]] ( [[Chiba/Engeneering_Flagella|1.Affinity Tag]] | [[Chiba/Communication|2.Communication Module]] | [[Chiba/Quorum_Sensing|3.Size Control]] ) |  [[Chiba/Making Marimo|Making Marimos]] |  [[Chiba/Goal|Our Goal]]</span><br>
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[[Chiba/Acknowledgements|Acknowledgements]] | [[Chiba/Team_Members|Team Members]] | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | [[Chiba/Members_Only|メンバ連絡簿]]  
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==Communication Unit==
==Communication Unit==
===Our Aim===
===Our Aim===
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[[Image:Chiba_prj_com.png|frame|'''Fig1.''' Micro.]]
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[[Image:Chiba_prj_com.png|frame|'''Fig. 13''' Quorum sensing for activating receivers!]]
Our aim is to make bacteria stick around a bacteria core.  
Our aim is to make bacteria stick around a bacteria core.  
Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal.
Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal.
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===About Quorum Sensing===
===About Quorum Sensing===
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[[Image:Chiba_aboutqs.png|frame|'''Fig2.''']]
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[[Image:Chiba_aboutqs.png|frame|'''Fig. 14''' Constructed circuit of Quorum sensing in our system.]]
Quorum sensing is a cell-to-cell signaling action of bacteria.
Quorum sensing is a cell-to-cell signaling action of bacteria.
They detect the cell density of the same species and coordinate the expression behavior of their cells.
They detect the cell density of the same species and coordinate the expression behavior of their cells.
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Species of Gram-Negativfe signaling transfer molecule (autoinducer) is acyl homoserine lactone (AHL). When the AHL concentration exceeded the threshold, the cell population is over a certain number, the AHL induced promoter plux transcription is activated.
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Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.
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Detail geneticaly circuit discribed in Fig2.
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Detailed gene circuit is discribed in Fig. 2.
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クオラムセンシングとは，バクテリアが細胞間で行うシグナル伝達のシステムである．自分と同種の菌の生息密度を感知して、それに応じて物質の産生をコントロールする．情報伝達に使用している化合物（オートインデューサー）はグラム陰性菌の場合AHLである．AHLの濃度がある値以上になる，すなわち細胞密度がある値を超えるとAHL依存性プロモータ（LuxP）のスイッチがオンになり，遺伝子が発現される．
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詳しい遺伝子回路は図に示す通り．
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<br clear="all">
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*more about quorum sensing
*more about quorum sensing
[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]
[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]
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==Experiments: New Parts!!==
==Experiments: New Parts!!==
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===Making Recievers===
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===Recievers===
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====New Parts We Made====
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====New Parts We Made!!====
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*ver.1(BBa_T729006)
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*AHL > GFP generator (constitutive aiiA) : [http://partsregistry.org/Part:BBa_I729006 BBa_T729006]
**[[Image:BBa I729006 circuit.jpg]]
**[[Image:BBa I729006 circuit.jpg]]
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*ver2.(BBa_I729005)
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*AHL > GFP & aiiA generator : [http://partsregistry.org/Part:BBa_I729005 BBa_I729005]
**[[Image:BBa I729005 circuit.jpg]]
**[[Image:BBa I729005 circuit.jpg]]
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*Sensitive AHL > GFP generator : [http://partsregistry.org/Part:BBa_I729004 BBa_I729004]
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**[[Image:Chiba_BBa_I729004.png]]
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====From Biobrick====
====From Biobrick====
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*ver.0(BBa_T9002)
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*BBa_T9002
**[[Image:T9002 circuit.jpg]]
**[[Image:T9002 circuit.jpg]]
====Future Work====
====Future Work====
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*ver3
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*inverter-aiiA
**[[Image:Rec inv aiia.jpg|357x40px]]
**[[Image:Rec inv aiia.jpg|357x40px]]
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===Making Senders===
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===Senders===
====New Parts We Made====
====New Parts We Made====
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*MetK Sender
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*MetK Sender ( could not deposit to registry; sequencing half completed )
[[Image:MetK circuit.jpg]]
[[Image:MetK circuit.jpg]]
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====From Biobrick====
====From Biobrick====
*Sender(BBa_S03623)
*Sender(BBa_S03623)
[[Image:BBa s03623.jpg]]
[[Image:BBa s03623.jpg]]
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====Future Work====
 
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*Light Sensing Sender (Light -> AHL)
 
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[[Image:Light sensing sender.jpg]]
 
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futureでLuxIもスイッチできた方が魅力的だと思うんだ。∵ReceiverにSenderを混ぜるのではなく，初めからReceiverとSenderを混ぜた状態で凝集させることができる。ex.)Photoswitch etc... by tashiro
 
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==Experiments==
==Experiments==

Latest revision as of 05:37, 27 October 2007

Chiba logo.png

Home
Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal
Acknowledgements | Team Members | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | メンバ連絡簿


Communication Unit

Our Aim

Fig. 13 Quorum sensing for activating receivers!

Our aim is to make bacteria stick around a bacteria core. Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal. Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags. Then they stick together. We use Quorum sensing as a signal.

About Quorum Sensing

Fig. 14 Constructed circuit of Quorum sensing in our system.

Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.

Detailed gene circuit is discribed in Fig. 2.

  • more about quorum sensing

[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]

[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]

Experiments: New Parts!!

Recievers

New Parts We Made!!

  • AHL > GFP generator (constitutive aiiA) : [http://partsregistry.org/Part:BBa_I729006 BBa_T729006]
    • BBa I729006 circuit.jpg
  • AHL > GFP & aiiA generator : [http://partsregistry.org/Part:BBa_I729005 BBa_I729005]
    • BBa I729005 circuit.jpg
  • Sensitive AHL > GFP generator : [http://partsregistry.org/Part:BBa_I729004 BBa_I729004]
    • Chiba BBa I729004.png

From Biobrick

  • BBa_T9002
    • T9002 circuit.jpg

Future Work

  • inverter-aiiA
    • Rec inv aiia.jpg

Senders

New Parts We Made

  • MetK Sender ( could not deposit to registry; sequencing half completed )

MetK circuit.jpg

From Biobrick

  • Sender(BBa_S03623)

BBa s03623.jpg

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