Edinburgh/DivisionPopper/Conclusions

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We have designed a switch that works as a function of bacterial division. This switch is naturally oscillatory, as opposed to other engineered switches. Switch frequency depends on what strain you place the device in. The ''dif'' sites are recombinatorial solutions that are employed universally by bacteria, so the PoPper should function well outwith the ''E. coli'' scope.
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We have designed a device called Division PoPper that works as a function of bacterial division. The device reports when a bacterial division happens by generating a PoPS pulse on a single copy plasmid inserted into the bacteria.
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We designed and implemented it by following the paradigms of Synthetic Biology approach and we also designed a Proof of Concept device for proving the validity of the main assumptions and mechanisms.
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The Divison PoPper has been a collected team effort. At the time of writing, we're still waiting for the Division Popper to arrive from GeneArt. Delivery has now been delayed by over a month and it looks like final piece in this jigsaw puzzle will be left out for now. The project does not end here, but will be continued as a final year dissertation project by a student, but we would have liked to present results.
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The most important mechanism to test is related to the dif sites, recombinatorial solutions that are employed universally by bacteria.
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We modelled both devices using classical and stochastic techniques and showed the quantitative and qualitative possibility of costructing such devices and composing it with a counter device.
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All the research we did into the topic still allows us to visualise the system outcome. The computer models show our predictions.
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In the wet lab we designed and constructed a total of thirteen biobricks that we registered and commented in the Registry. We propose our device as interesting example of conversion from a meaningful biological process to a standard logical signal. The device can be used for controlling cell division frequency or trigger actions related to division counting.
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This wiki will be updated until the very moment it freezes, so please watch this space. We keep our fingers crossed.
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[[Image:Popper.JPG]]
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Please take some time to visit [http://www.macteria.co.uk/popper.php This page ] for an interactive Flash animation of the final construct!
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We look forward to meeting you all at the Jamboree.
We look forward to meeting you all at the Jamboree.

Latest revision as of 05:00, 27 October 2007

MENU : Introduction | Background | Applications | Design&Implementation | Modelling | Wet Lab | Synthetic Biology Approach | Conclusions


We have designed a device called Division PoPper that works as a function of bacterial division. The device reports when a bacterial division happens by generating a PoPS pulse on a single copy plasmid inserted into the bacteria. We designed and implemented it by following the paradigms of Synthetic Biology approach and we also designed a Proof of Concept device for proving the validity of the main assumptions and mechanisms. The most important mechanism to test is related to the dif sites, recombinatorial solutions that are employed universally by bacteria. We modelled both devices using classical and stochastic techniques and showed the quantitative and qualitative possibility of costructing such devices and composing it with a counter device. In the wet lab we designed and constructed a total of thirteen biobricks that we registered and commented in the Registry. We propose our device as interesting example of conversion from a meaningful biological process to a standard logical signal. The device can be used for controlling cell division frequency or trigger actions related to division counting.

We look forward to meeting you all at the Jamboree.

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