Wisconsin/Protocol:Agarose Gel
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*1uL 10mg/ml EtBr | *1uL 10mg/ml EtBr | ||
| - | Mix agarose and TBE in flask, microwave to make sure agarose is dissolved in TBE. Add EtBr and pour into gel box. | + | Mix agarose and TBE in flask, microwave for 20 second intervals and swirl it until solution is clear (usually 1-2min. total) to make sure agarose is dissolved in TBE. Add EtBr to flask (under hood) and pour into gel box with comb in place. Wait until gel solidifies. |
*2uL DNA | *2uL DNA | ||
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*4uL DNA Ladder for reference | *4uL DNA Ladder for reference | ||
| - | Add water if DNA is less than 10uL and make sure #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA. | + | Add water if DNA is less than 10uL and make sure #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA. Run to red half way. |
Latest revision as of 17:20, 6 July 2007
1.2% Agarose Gel preparation
- 8 wells
- 600mg Agarose
- 50mL TBE
- 1uL 10mg/ml EtBr
Mix agarose and TBE in flask, microwave for 20 second intervals and swirl it until solution is clear (usually 1-2min. total) to make sure agarose is dissolved in TBE. Add EtBr to flask (under hood) and pour into gel box with comb in place. Wait until gel solidifies.
- 2uL DNA
- 12uL ddH2O
- 4uL 5x Loading Dye
- 4uL DNA Ladder for reference
Add water if DNA is less than 10uL and make sure #uL of 1x loading dye = #uL total. Mix content in tube before adding it to well. Also make sure gel box is submerged in TAE/TBE before adding DNA. Run to red half way.
