BerkiGEM2007-AustinConstructionFile21
From 2007.igem.org
(Difference between revisions)
(One intermediate revision not shown) | |||
Line 1: | Line 1: | ||
Alpha: <br> | Alpha: <br> | ||
PCR CA1150/CA1099R on 9145-I716070 (1727 Bp)<br> | PCR CA1150/CA1099R on 9145-I716070 (1727 Bp)<br> | ||
- | Digest (Bsa1/Bgl2) and ligate with beta portion.<br> <br> | + | Digest (Bsa1/Bgl2) (1686/Small, Larger) and ligate with beta portion.<br> <br> |
Beta: <br> | Beta: <br> | ||
- | PCR CA1099F/G00101 on Part 9145-I716080 <br> | + | PCR CA1099F/G00101 on Part 9145-I716080 (1567 bp)<br> |
- | Digest (Bsa1/BamH1) and ligate with alpha portion. <br> | + | Digest (Bsa1/BamH1) (1441/small, Larger) and ligate with alpha portion. <br> |
<br><br> | <br><br> | ||
Product is 9145-I716071 | Product is 9145-I716071 |
Latest revision as of 22:45, 10 July 2007
Alpha:
PCR CA1150/CA1099R on 9145-I716070 (1727 Bp)
Digest (Bsa1/Bgl2) (1686/Small, Larger) and ligate with beta portion.
Beta:
PCR CA1099F/G00101 on Part 9145-I716080 (1567 bp)
Digest (Bsa1/BamH1) (1441/small, Larger) and ligate with alpha portion.
Product is 9145-I716071