Melbourne/primary Restriction enzymes

From 2007.igem.org

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|-
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!Enzyme
!Enzyme
-
!
+
!Cut site
 +
!100% buffers
!Cost
!Cost
!No of U
!No of U
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!location
!location
!In store
!In store
 +
|-
 +
!SpeI
 +
|5A^CTAGT3
 +
|B2
 +
|US$244
 +
|2500U @ 10,000U/ml
 +
|[http://www.neb.com/nebecomm/products/productR0140.asp NEB.R0133L]
 +
|
 +
|
 +
|-
 +
!HindIII
 +
|5A^AGCTT3
 +
|B2
 +
|US$53
 +
|10,000 @ 20,000U/ml
 +
|[http://www.neb.com/nebecomm/products/productR0140.asp NEB.R0104S]
 +
|
 +
|
|-
|-
!PstI
!PstI
|5CTGCA^G3
|5CTGCA^G3
 +
|B3
|US$58
|US$58
|10,000 @ 20,000U/ml
|10,000 @ 20,000U/ml
-
|[[http://www.neb.com/nebecomm/products/productR0140.asp|NEB.R0140S]]
+
|[http://www.neb.com/nebecomm/products/productR0140.asp NEB.R0140S]
-
|
+
|[[Media:Melbourne Lab Enzyme Freezer.jpg|Lab Enz Freezer Box]]
|YES
|YES
|-
|-
!EcoRI
!EcoRI
|5G^AATTC3
|5G^AATTC3
-
|
+
|B1,2,3&4
-
|
+
|AU$89
-
|[[http://www.neb.com/nebecomm/products/productR0101.asp|NEB.R0101S]]
+
|10,000 @ 20,000U/ml
-
|
+
|[http://www.neb.com/nebecomm/products/productR0101.asp NEB.R0101S]
 +
|[[Media:Melbourne Lab Enzyme Freezer.jpg|Lab Enz Freezer Box]]
|YES
|YES
|-
|-
-
!HaeI
+
!HaeII
-
|
+
|5RGCGC^Y3
-
|
+
|B4 & B2
-
|
+
|US$53
-
|
+
|2,000U @ 20,000U/ml
 +
|[http://www.neb.com/nebecomm/products/productR0107.asp NEB.R0107S]
 +
|[[Media:Melbourne Igem Freezer|Igem Freezer box]]
|YES
|YES
|-
|-
!SalI
!SalI
-
|
+
|5G^TCGAC3
-
|
+
|B3
-
|
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|US$56
-
|
+
|2,000U @ 20,000U/ml
 +
|[http://www.neb.com/nebecomm/products/productR0138.asp NEB.R0138S]
 +
|[[Media:Melbourne Lab Enzyme Freezer.jpg|Lab Enz Freezer Box]]
|YES
|YES
|-
|-
!XbaI
!XbaI
-
|
+
|5T^CTAGA3
-
|
+
|B2
-
|
+
|US$63
-
|
+
|3,000U @ 20,000U/ml
 +
|[http://www.neb.com/nebecomm/products/productR0145.asp NEB.R0145S]
 +
|[[Media:Melbourne Lab Enzyme Freezer.jpg|Lab Enz Freezer Box]]
|YES
|YES
|}
|}
 +
Y= (C or T), R= (A or G)
 +
[http://www.neb.com/nebecomm/tech_reference/single_code.asp Single letter code]
 +
 +
====Double Digest Buffers====
 +
 +
 +
====Lab Location====
====Lab Location====
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====Store====
====Store====
-
*Available from Bio21 store? Yes  
+
*Available from Bio21 store? Yes
 +
*location: [Media:melbourne Erefrigerator |E-refrigerator] Needs account card. (see [[Media:melbourne daisy |Daisy]]).
-
====Supplier:====
 
-
*Supplier:
 
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*Suppliers part no:
 
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*Contact details for supplier:
 
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*Email:
 
-
*Phone:
 
====Manufacturer:====
====Manufacturer:====

Latest revision as of 10:58, 2 August 2007

<return to list of primary reagents> <team home page>

Contents

Physical data

  • Description: NEB Restriction enzymes are used generally in the Lab.
NEB restriction enzymes
Enzyme Cut site 100% buffers Cost No of U Part no/data location In store
SpeI 5A^CTAGT3 B2 US$244 2500U @ 10,000U/ml [http://www.neb.com/nebecomm/products/productR0140.asp NEB.R0133L]
HindIII 5A^AGCTT3 B2 US$53 10,000 @ 20,000U/ml [http://www.neb.com/nebecomm/products/productR0140.asp NEB.R0104S]
PstI 5CTGCA^G3 B3 US$58 10,000 @ 20,000U/ml [http://www.neb.com/nebecomm/products/productR0140.asp NEB.R0140S] Lab Enz Freezer Box YES
EcoRI 5G^AATTC3 B1,2,3&4 AU$89 10,000 @ 20,000U/ml [http://www.neb.com/nebecomm/products/productR0101.asp NEB.R0101S] Lab Enz Freezer Box YES
HaeII 5RGCGC^Y3 B4 & B2 US$53 2,000U @ 20,000U/ml [http://www.neb.com/nebecomm/products/productR0107.asp NEB.R0107S] Igem Freezer box YES
SalI 5G^TCGAC3 B3 US$56 2,000U @ 20,000U/ml [http://www.neb.com/nebecomm/products/productR0138.asp NEB.R0138S] Lab Enz Freezer Box YES
XbaI 5T^CTAGA3 B2 US$63 3,000U @ 20,000U/ml [http://www.neb.com/nebecomm/products/productR0145.asp NEB.R0145S] Lab Enz Freezer Box YES

Y= (C or T), R= (A or G) [http://www.neb.com/nebecomm/tech_reference/single_code.asp Single letter code]

Double Digest Buffers

Lab Location

  • Location see table
  • Permision to use required? No


Store

  • Available from Bio21 store? Yes
  • location: [Media:melbourne Erefrigerator |E-refrigerator] Needs account card. (see Daisy).


Manufacturer:




  • PhillipDodson 03:48, 1 July 2007 (EDT):Primary Reagent Template 1/July/2007
Retrieved from "http://2007.igem.org/wiki/index.php/Melbourne/primary_Restriction_enzymes"