Glasgow/Wetlab/Protocols
From 2007.igem.org
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| - | == Transforming BioBricks | + | == Transforming BioBricks == |
#9 reactions including 5 tubes of competent cells (4 x Top 10, 1 x DB3.1). | #9 reactions including 5 tubes of competent cells (4 x Top 10, 1 x DB3.1). | ||
#split the content (~75ul) in two. | #split the content (~75ul) in two. | ||
Revision as of 17:24, 19 July 2007
Transforming BioBricks
- 9 reactions including 5 tubes of competent cells (4 x Top 10, 1 x DB3.1).
- split the content (~75ul) in two.
- competent cells (~33ul) into eppendorfs on ice..
- add 1ul of plasmid DNA.
- incubate on ice for at least 5 mins.
- heat shock +42 ºC for 45 secs.
- back on ice for 2 mins to reduce damages.
- add 1ml of soc media.
- incubate +37ºC in a shaker for 1 hour.
- spread the reaction on kan/carb LB plates.
- grow +37 ºC overnight.
