Glasgow/Wetlab/Orders

From 2007.igem.org

(Difference between revisions)
(→Emma's primers)
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!Tm
!Tm
|-
|-
-
|*PHZ-UP
+
|*PhZ-UP
|5' TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
|5' TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
|65.6
|65.6
|-
|-
-
|*PHZ-LOW
+
|*PhZ-LOW
|5' CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CA G 3'
|5' CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CA G 3'
|64.8
|64.8
|-
|-
-
|*bbpPHZ_Up
+
|*BbpPhZ_Up
|5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
|5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
|63.6
|63.6
|-
|-
-
|*bbsPHZ_Low
+
|*BbsPhZ_Low
|5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
|5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
|64.2
|64.2
|-
|-
-
|bbpgene
+
|BbpGene
|5' gaa ttc gcg gcc gct tct ag 3'
|5' gaa ttc gcg gcc gct tct ag 3'
|65.7
|65.7
|-
|-
-
|bbs
+
|Bbs
|5' ctg cag cgg ccg cta cta gta 3'
|5' ctg cag cgg ccg cta cta gta 3'
|65.3
|65.3
|-
|-
-
|
+
|*METHYL1
-
|
+
|5' TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3'
-
|
+
|66.3
-
|
+
|-
-
|
+
|*METHYL2
-
|
+
|5' TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3'
-
|
+
|66.2
-
|
+
|-
-
|
+
|*BbpMethyl1
-
|
+
|5' gaa ttc gcg gcc gct tct aga TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3'
-
|
+
|63.9
-
|
+
|-
-
|
+
|*BbsMethyl2
-
|
+
|5' ctg cag cgg ccg cta cta gta TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3'
-
|
+
|66.9
-
|
+
|-
-
|
+
|PhzMfor1
-
|
+
|5' atg aat aat tcg aat ctt gct gct 3'
-
|
+
|65.4
 +
|-
 +
|PhzMrev1
 +
|5' tca ggc cct ggc agc gac 3'
 +
|60.3
 +
|-
 +
|BbpPhzMfor1
 +
|5' gaa ttc gcg gcc gct tct aga tga ata att cga atc ttg ctg ct 3'
 +
|64.5
 +
|-
 +
|BbsPhzMrev1
 +
|5' ctg cag cgg ccg cta cta gta tca ggc cct ggc agc gac 3'
 +
|60.3
 +
|-
 +
|*OXY1
 +
|5' TCG AAC ACT CTA GAA AAG GAA GCA CC 3'
 +
|60.2
 +
|-
 +
|*OXY2
 +
|5' TTT TTT GCA TGC TAG CGT GGC CGT TCC 3'
 +
|59.2
 +
|-
 +
|*BbpOxy1
 +
|5' gaa ttc gcg gcc gct tct aga TCG AAC ACT CTA GAA AAG GAA GCA CC 3'
 +
|65.1
 +
|-
 +
|*BbsOxy2
 +
|5' ctg cag cgg ccg cta cta gta TTT TTT GCA TGC TAG CGT GGC CGT TCC 3'
 +
|66.9
 +
|-
 +
|PhzSfor1
 +
|5' atg agc gaa ccc atc gat at 3'
 +
|71.2
 +
|-
 +
|PhzSrev1
 +
|5' cta gcg tgg ccg ttc cac ctg 3'
 +
|60.2
 +
|-
 +
|BbpPhzSfor1
 +
|5' gaa ttc gcg gcc gct tct aga tga gcg aac cca tcg ata t 3'
 +
|70.9
 +
|-
 +
|BbsPhzSrev1
 +
|5' ctg cag cgg ccg cta cta gta cta gcg tgg ccg ttc cac ctg 3'
 +
|63.3
 +
|-
 +
|PhzH_up
 +
|5' CGC ACG GAT CCT TTC AGA ATG TTC 3'
 +
|69.9
 +
|-
 +
|PhzH_low
 +
|5' GCC ACG CCA AGC TTC ACG CTC A 3'
 +
|70.6
 +
|-
 +
|BbpPhZ_Up
 +
|5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
 +
|63.3
 +
|-
 +
|BbsPhZ-Low
 +
|5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
 +
|57.7
 +
|}

Revision as of 13:21, 24 July 2007

Glasgow Main Page

Emma's primers

Name Sequence Tm
*PhZ-UP 5' TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3' 65.6
*PhZ-LOW 5' CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CA G 3' 64.8
*BbpPhZ_Up 5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3' 63.6
*BbsPhZ_Low 5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3' 64.2
BbpGene 5' gaa ttc gcg gcc gct tct ag 3' 65.7
Bbs 5' ctg cag cgg ccg cta cta gta 3' 65.3
*METHYL1 5' TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3' 66.3
*METHYL2 5' TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3' 66.2
*BbpMethyl1 5' gaa ttc gcg gcc gct tct aga TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3' 63.9
*BbsMethyl2 5' ctg cag cgg ccg cta cta gta TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3' 66.9
PhzMfor1 5' atg aat aat tcg aat ctt gct gct 3' 65.4
PhzMrev1 5' tca ggc cct ggc agc gac 3' 60.3
BbpPhzMfor1 5' gaa ttc gcg gcc gct tct aga tga ata att cga atc ttg ctg ct 3' 64.5
BbsPhzMrev1 5' ctg cag cgg ccg cta cta gta tca ggc cct ggc agc gac 3' 60.3
*OXY1 5' TCG AAC ACT CTA GAA AAG GAA GCA CC 3' 60.2
*OXY2 5' TTT TTT GCA TGC TAG CGT GGC CGT TCC 3' 59.2
*BbpOxy1 5' gaa ttc gcg gcc gct tct aga TCG AAC ACT CTA GAA AAG GAA GCA CC 3' 65.1
*BbsOxy2 5' ctg cag cgg ccg cta cta gta TTT TTT GCA TGC TAG CGT GGC CGT TCC 3' 66.9
PhzSfor1 5' atg agc gaa ccc atc gat at 3' 71.2
PhzSrev1 5' cta gcg tgg ccg ttc cac ctg 3' 60.2
BbpPhzSfor1 5' gaa ttc gcg gcc gct tct aga tga gcg aac cca tcg ata t 3' 70.9
BbsPhzSrev1 5' ctg cag cgg ccg cta cta gta cta gcg tgg ccg ttc cac ctg 3' 63.3
PhzH_up 5' CGC ACG GAT CCT TTC AGA ATG TTC 3' 69.9
PhzH_low 5' GCC ACG CCA AGC TTC ACG CTC A 3' 70.6
BbpPhZ_Up 5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3' 63.3
BbsPhZ-Low 5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3' 57.7
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