Paris/Oligos
From 2007.igem.org
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| ForDGAT1 | | ForDGAT1 | ||
| - | | | + | | ATCCACGCTATGCGCCCATTAC |
| - | | | + | | 22 |
| - | | | + | | Tm = 60°C |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" | 28 | | style="background: #ccffcc;" | 28 | ||
| RevDGAT1 | | RevDGAT1 | ||
| - | | | + | | GGGCTAATTTAGCCCTTTAGTT |
| - | | | + | | 22 |
| - | | | + | | Tm = 50°C |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" | 29 | | style="background: #ccffcc;" | 29 | ||
| ForDGAT_BB1 | | ForDGAT_BB1 | ||
| - | | | + | | AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATGCGCCCATTAC |
| - | | | + | | 62 |
| - | | | + | | Tm = 60/83°C |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" | 30 | | style="background: #ccffcc;" | 30 | ||
| RevDGAT_BB1 | | RevDGAT_BB1 | ||
| - | | | + | | CTGCAGCGGCCGCTACTAGTAGGGCTAATTTAGCCCTTTAGTT |
| - | | | + | | 43 |
| - | | | + | | Tm = 50/74°C |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
Revision as of 13:08, 30 July 2007
| Table of Oligos | ||||
|---|---|---|---|---|
| Number | Name | Sequence | Length | Comments |
| 1 | Lox71-FtsZ-F | TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT | 89 | |
| 2 | FtsZ-R | TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT | 49 | |
| 3 | Lox71-FtsA-F | TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACG AAGTTATTAATAATCAGGCACAGGCAGAACAACAATG | 89 | |
| 4 | DEcoR1-FtsZ-R | CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC | 40 | |
| 5 | DEcoR1-FtsZ-F | TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA | 40 | |
| 6 | Lox66-DapAColi-F | TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAGTTTGCACAGAGGATGGCCCATGTT | 84 | |
| 7 | DapAColi-R | TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC | 52 | |
| 8 | Lox66-DapASubtilis-F | TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAATCATGATGAGGTGAATAAAATGAA | 84 | |
| 9 | DapASubtilis-R | TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT | 52 | |
| 10 | Lox71-KmR-F | TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACG AAGTTATCTGATCCTTCAACTCAGCAAAAGTTC | 85 | |
| 11 | Lox66-KmR-R | TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCA AGTTATTTATTAGAAAAACTCATCGAGCATCAAA | 86 | |
| 12 | DPst1-DGAT-F | AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT | 40 | |
| 13 | DPst1-DGAT-R | AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT | 40 |
|
| 14 | Lox66-F | AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTATAC TAGTAGCGGCCGCTGCA | ||
| 15 | Lox66-R | GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATCTCTAG AAGCGGCCGCG | ||
| 16 | Lox71-F | AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTACT AGTAGCGGCCGCTGCA | ||
| 17 | Lox71-R | GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTACTCTAG AAGCGGCCGCG | ||
| 18 | VF2 | tgccacctgacgtctaagaa | 20 | Forward Primer to Biobrick vectors (VF2) BBa_G00100 |
| 19 | VR | attaccgcctttgagtgagc | 20 | Reverse Primer to Biobrick Vectors (VR) BBa_G00101 |
| 20 | RBS-DapAColi-F | TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGTT CACGGGAAGTATTGTCGCGA | 71 | Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Coli) from ATG |
| 21 | RBS-DapASubtilis-F | TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGAA TTTCGGAAATGTGTCTACAG | 71 | Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Subtilis) from ATG |
| 22 | SEQFtsA-1F | GTCGTCCATACCGCGAAATC | 20 | |
| 23 | SEQFtsA-2F | CGTGGAAGTGCCGAGCGTAG | 20 | |
| 24 | SEQFtsZ-1F | GGTATCGCTGAACTGATTAC | 20 | |
| 25 | SEQDapASub-1F | TTAAAGCAATTGCGGCAGAG | 20 | |
| 26 | SEQDapAColi-1F | CAATCGTCCGTCGCAAGAAG | 20 | |
| 27 | ForDGAT1 | ATCCACGCTATGCGCCCATTAC | 22 | Tm = 60°C |
| 28 | RevDGAT1 | GGGCTAATTTAGCCCTTTAGTT | 22 | Tm = 50°C |
| 29 | ForDGAT_BB1 | AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATGCGCCCATTAC | 62 | Tm = 60/83°C |
| 30 | RevDGAT_BB1 | CTGCAGCGGCCGCTACTAGTAGGGCTAATTTAGCCCTTTAGTT | 43 | Tm = 50/74°C |
| 31 | DapAp-F | aattcgcggccgcttctagagAACAATCAGAACGGTTCTGTCTGCTTGCTTTT AATGCCATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGATGGta | 104 | |
| 32 | DapAp-R | ctagtaCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCA TTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTctctagaagcggccgcg | 104 | |
| 33 | dDapA-F | CATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGAT GGCCCATGATTCCGGGGATCCGTCGACC | 70 | more here |
| 34 | dDapA-R | AAGCCATCAAATCTCCCTAAACTTTACAGCAAACCGGCATGC TTAAGCGCTGTAGGCTGGAGCTGCTTCG | 70 | more here |
| 35 | dFtsZ-F | GACGATGATTACGGCCTCAGGCGACAGGCACAAATCGGAGAGA AACTATGATTCCGGGGATCCGTCGACC | 70 | more here |
| 36 | dFtsZ-R | GAAACCCAAATTCCAGTCAATTCTTAATCAGCTTGCTTACGCA GGAATGCTGTAGGCTGGAGCTGCTTCG | 70 | more here |
| 37 | dDapAverif-F | ACAATCAGAACGGTTCTGTCTG | 22 | |
| 38 | dDapAverif-R | ATAGCGTGAGTCAGAACTACAG | 22 | |
| 39 | dFtsZverif-R | TCGCCAAATTACCTATCCAACC | 22 | |
