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Revision as of 10:50, 3 August 2007

1. To prepare 1 l of SOC medium dissolve in ultrapure water:

-Tryptone 4 g

-Yeast extract 1g

-1M NaCl 2 ml

-1M KCl 0.5 ml

-5M NaOH 200 μl to adjust the pH to 6.8

2 After autoclave add 2 ml each of 2M Mg-salt (1M MgSO4 and 1M MgCl) and 2M glucose.

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