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	::1. Mix :		::1. Mix :
	::::: - 1 μg of plasmid		::::: - 1 μg of plasmid
-	::::: - 3 μl of Buffer 10X	+	::::: - 3 μl of Buffer 10X [ ]
-	::::: - 0.3 μl BSA 100X	+	::::: - 0.3 μl BSA 100X [ ]
	::::: - 3 units of restriction enzyme		::::: - 3 units of restriction enzyme
	::::: - water up to 30 μl		::::: - water up to 30 μl

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Revision as of 09:30, 25 October 2007

(final volume 30μl)

1. Mix :

- 1 μg of plasmid

- 3 μl of Buffer 10X [ ]

- 0.3 μl BSA 100X [ ]

- 3 units of restriction enzyme

- water up to 30 μl

2. Incubate at 37 °C for 1 hr.

3. Incubate at the inactivation temperature of the enzyme for 20 minutes.

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