Bologna University/Ligation reaction

(Difference between revisions)

Revision as of 15:12, 23 August 2007

(final volume 20 μl)

1. Mix :

- 8 μl of insert DNA

- 4 μl of vector plasmid

- 4 μl of Buffer 5X

- 1 μl of T4 Ligase

- 3 μl of mQ water

2. Incubate at 4 °C overnigth or at 15 °C for 3 hr.

@@ Line 1: / Line 1: @@
-:: Not yet setted
+:: (final volume 20 μl)
+::1. Mix :
+::::: - 8 μl of insert DNA
+::::: - 4 μl of vector plasmid
+::::: - 4 μl of Buffer 5X
+::::: - 1 μl of T4 Ligase
+::::: - 3 μl of mQ water
+::2. Incubate at 4 °C overnigth or at 15 °C for 3 hr.
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