Week 2
From 2007.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
We started plasmid amplification from IGEM 2007 plates. | We started plasmid amplification from IGEM 2007 plates. | ||
| - | We had some problems with Ampicillin solubility in water. We found that our Ampicillin was not soluble in water, so we got to try to resuspend it in NaOH 1M with a work concentration of 50 | + | We had some problems with Ampicillin solubility in water. We found that our Ampicillin was not soluble in water, so we got to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we tried to change the antibiotic. |
| - | So, we used a water- soluble Ampicillin with a concentration of 100 | + | So, we used a water- soluble Ampicillin with a concentration of 100 μg/ml. We found that it finally worked. |
[[Bologna | Back]] | [[Bologna | Back]] | ||
Revision as of 10:32, 6 August 2007
- 9/07/07 - 15/07/07
We started plasmid amplification from IGEM 2007 plates.
We had some problems with Ampicillin solubility in water. We found that our Ampicillin was not soluble in water, so we got to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we tried to change the antibiotic. So, we used a water- soluble Ampicillin with a concentration of 100 μg/ml. We found that it finally worked.
