Week 2
From 2007.igem.org
(Difference between revisions)
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| - | We started plasmid amplification from IGEM 2007 plates. | + | *We started plasmid amplification from IGEM 2007 plates. |
| - | We had some problems with Ampicillin solubility in water. We found that our Ampicillin was not soluble in water, so we got to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we tried to change the antibiotic. | + | *We had some problems with Ampicillin solubility in water. We found that our Ampicillin was not soluble in water, so we got to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we tried to change the antibiotic. |
So, we used a water- soluble Ampicillin with a concentration of 100 μg/ml. We found that it finally worked. | So, we used a water- soluble Ampicillin with a concentration of 100 μg/ml. We found that it finally worked. | ||
| + | |||
| + | *We also had some problems with Kanamycin. We first used it at a work concentration of 20 g/ml, but it didn’t work. Guys from the Boston University team had been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it worked correctly. | ||
| + | |||
| + | *Preparation of IPTG stocks 100 mM (collegamento al protocollo) | ||
| + | |||
[[Bologna | Back]] | [[Bologna | Back]] | ||
Revision as of 10:34, 6 August 2007
- 9/07/07 - 15/07/07
- We started plasmid amplification from IGEM 2007 plates.
- We had some problems with Ampicillin solubility in water. We found that our Ampicillin was not soluble in water, so we got to try to resuspend it in NaOH 1M with a work concentration of 50 μg/ml. Since bacterial cells still grew on our plates, we tried to change the antibiotic.
So, we used a water- soluble Ampicillin with a concentration of 100 μg/ml. We found that it finally worked.
- We also had some problems with Kanamycin. We first used it at a work concentration of 20 g/ml, but it didn’t work. Guys from the Boston University team had been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it worked correctly.
- Preparation of IPTG stocks 100 mM (collegamento al protocollo)
