BerkiGEM2007-AustinConstructionFile54
From 2007.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
Quickchange AD0015F/R to get rid of XhoI site. <br> | Quickchange AD0015F/R to get rid of XhoI site. <br> | ||
<br> | <br> | ||
| - | PCR AD0016F/R on modified I716091. | + | PCR AD0016F/R on modified I716091.<br> |
| - | Digest Bgl2/Xho1 and insert into 9145 plasmid. | + | Digest Bgl2/Xho1 and insert into 9145 plasmid.<br> |
| + | Product is I716354<br> | ||
Latest revision as of 01:50, 9 August 2007
Pre-biobricking modifications to I716091.
Quickchange AD0013F/R to get rid of EcoR1 site.
Quickchange AD0014/F/R to get rid of BglII site.
Quickchange AD0015F/R to get rid of XhoI site.
PCR AD0016F/R on modified I716091.
Digest Bgl2/Xho1 and insert into 9145 plasmid.
Product is I716354
