Melb:Plan:AND Gate
From 2007.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
#If positive ligate (overnight) | #If positive ligate (overnight) | ||
#Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight) | #Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight) | ||
| - | #Miniprep and digest to confirm and glycerol stocks. | + | #Miniprep and digest to confirm and glycerol stocks. (1 day) |
| + | |||
| + | Next need to ligate to RBS-ComA-Ter construct. | ||
Latest revision as of 08:08, 11 August 2007
- Retransform c1 inverter (afternoon + overnight)
- Liquid culture (overnight)
- Isolate plasmid (mini prep kit not working - to big? - best way to isolate?) (half day)
- Confirm Isolation and part using XbaI/PstI digest (half day)
- If feeling optimistic digest OmpR promoter simultaneously with SpeI/PstI and prepare to gel purify fragments of OmpR promoter (vector) and the inverter(insert)
- If positive ligate (overnight)
- Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight)
- Miniprep and digest to confirm and glycerol stocks. (1 day)
Next need to ligate to RBS-ComA-Ter construct.
