Glasgow/Wetlab/Orders

From 2007.igem.org

(Difference between revisions)
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== Designed primers ==
== Designed primers ==
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{| border="1" cellspacing="0" cellpadding="5" align="center"
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!Name
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!Sequence
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!Tm
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|-
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|DmpR_SDM_PstI_1_for
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|5´CTGGAGCAACAGCATGCGCTGTTC3´
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|65.6
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|-
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|DmpR_SDM_PstI_1_rev
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|5´GCTCCAGTTTTCAGCGATGGCC3´
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|64.8
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|-
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|DmpR_SDM_PstI_2_for
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|5´CTGGAGGAAGTGCTCAACCAGCAG3´
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|66.3
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|-
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|DmpR_SDM_PstI_2_rev
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|5´CCTCCAGCGCTTCCACCAG3´
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|66.2
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|-
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|DmpR_Start_Prefix_1_for
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|5´GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG3´
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|60.2
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|-
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|DmpR_End_Suffix_1_rev
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|5´CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC3´
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|59.2
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|-
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|***M_SDM_PstI_1_for
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|5´***3´
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|69.9
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|-
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|***M_SDM_PstI_1_rev
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|5´***3´
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|70.6
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|-
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|***S_SDM_PstI_1_for
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|5´***3´
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|65.7
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|-
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|***S_SDM_PstI_1_rev
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|5´***3´
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|65.3
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|-
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|***S_SDM_PstI_2_for
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|5´***3´
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|63.6
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|-
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|***S_SDM_PstI_2_rev
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|5´***3´
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|63.9
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|-
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|***C_SDM_PstI_1_for
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|5´***3´
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|65.1
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|-
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|***C_SDM_PstI_1_rev
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|5´***3´
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|64.2
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|-
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|***F_SDM_PstI_1_for
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|5´***3´
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|66.9
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|-
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|***F_SDM_PstI_1_rev
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|5´***3´
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|66.9
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|-
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|***B_SDM_EcoRI_1_for
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|5'***3'
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|65.4
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|-
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|***B_SDM_EcoRI_1_rev
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|5'***3'
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|64.5
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|-
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|***E_SDM_EcoRI_1_for
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|5'***3'
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|60.3
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|-
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|***E_SDM_EcoRI_1_rev
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|5'***3'
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|60.3
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|-
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|***E_SDM_PstI_1_for
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|5'***3'
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|71.2
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|-
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|***E_SDM_PstI_1_rev
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|5'***3'
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|70.9
 +
|-
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|XylR_SDM_PstI_1_for
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|5´GAGCTCCAGACCCAGGTTG3´
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|63.3
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|-
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|XylR_SDM_PstI_1_rev
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|5´GTCTGGAGCTCGTAGCGCTC3´
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|63.3
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|-
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|XylR_Prefix_1
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|5´GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG3´
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|57.7
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|-
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|XylR_Suffix_1
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|5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC3´
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|59.8
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|-
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|Pu_Prefix_1(AfterXylR)
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|5´GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG3´
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|65.0
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|-
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|Pu_Suffix_1(AfterXylR)
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|5´CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG3´
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|65.1
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|-
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|Pu_Prefix_2(Emma´s sequence)
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|5´GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC3´
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|66.0
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|-
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|Pu_Suffix_2(Emma´s sequence)
 +
|5´CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC3´
 +
|66.4
 +
|}

Revision as of 10:15, 16 August 2007

Glasgow Main Page

Emma's primers

Name Sequence Tm
*(***)-Up 5' *** C 3' 65.6
*(***)-Low 5' *** 3' 64.8
*Bbp_(***)_Up 5' *** 3' 63.6
*Bbs_(***)_Low 5' *** 3' 64.2
Bbp_Gene 5' gaa ttc gcg gcc gct tct ag 3' 65.7
Bbs 5' ctg cag cgg ccg cta cta gta 3' 65.3
*Methyl_1 5' *** 3' 66.3
*Methyl_2 5' *** 3' 66.2
*Bbp_Methyl_1 5' *** 3' 63.9
*Bbs_Methyl_2 5' *** 3' 66.9
(***)_M_for_1 5' *** 3' 65.4
(***)_M_rev_1 5' *** 3' 60.3
Bbp_(***)_M_for_1 5' *** 3' 64.5
Bbs_(***)_M_rev_1 5' *** 3' 60.3
*Oxy_1 5' *** 3' 60.2
*Oxy_2 5' *** 3' 59.2
*Bbp_Oxy_1 5' *** 3' 65.1
*Bbs_Oxy_2 5' *** 3' 66.9
(***)_S_for_1 5' *** 3' 71.2
(***)_S_rev_1 5' *** 3' 60.2
Bbp_(***)_S_for_1 5' *** 3' 70.9
Bbs_(***)_S_rev_1 5' *** 3' 63.3
(***)_H_up 5' *** 3' 69.9
(***)_H_low 5' *** 3' 70.6
Bbp_(***)_Up 5' *** 3' 63.3
Bbs_(***)_Low 5' *** 3' 57.7

Designed primers

Name Sequence Tm
DmpR_SDM_PstI_1_for 5´CTGGAGCAACAGCATGCGCTGTTC3´ 65.6
DmpR_SDM_PstI_1_rev 5´GCTCCAGTTTTCAGCGATGGCC3´ 64.8
DmpR_SDM_PstI_2_for 5´CTGGAGGAAGTGCTCAACCAGCAG3´ 66.3
DmpR_SDM_PstI_2_rev 5´CCTCCAGCGCTTCCACCAG3´ 66.2
DmpR_Start_Prefix_1_for 5´GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG3´ 60.2
DmpR_End_Suffix_1_rev 5´CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC3´ 59.2
***M_SDM_PstI_1_for 5´***3´ 69.9
***M_SDM_PstI_1_rev 5´***3´ 70.6
***S_SDM_PstI_1_for 5´***3´ 65.7
***S_SDM_PstI_1_rev 5´***3´ 65.3
***S_SDM_PstI_2_for 5´***3´ 63.6
***S_SDM_PstI_2_rev 5´***3´ 63.9
***C_SDM_PstI_1_for 5´***3´ 65.1
***C_SDM_PstI_1_rev 5´***3´ 64.2
***F_SDM_PstI_1_for 5´***3´ 66.9
***F_SDM_PstI_1_rev 5´***3´ 66.9
***B_SDM_EcoRI_1_for 5'***3' 65.4
***B_SDM_EcoRI_1_rev 5'***3' 64.5
***E_SDM_EcoRI_1_for 5'***3' 60.3
***E_SDM_EcoRI_1_rev 5'***3' 60.3
***E_SDM_PstI_1_for 5'***3' 71.2
***E_SDM_PstI_1_rev 5'***3' 70.9
XylR_SDM_PstI_1_for 5´GAGCTCCAGACCCAGGTTG3´ 63.3
XylR_SDM_PstI_1_rev 5´GTCTGGAGCTCGTAGCGCTC3´ 63.3
XylR_Prefix_1 5´GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG3´ 57.7
XylR_Suffix_1 5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC3´ 59.8
Pu_Prefix_1(AfterXylR) 5´GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG3´ 65.0
Pu_Suffix_1(AfterXylR) 5´CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG3´ 65.1
Pu_Prefix_2(Emma´s sequence) 5´GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC3´ 66.0
Pu_Suffix_2(Emma´s sequence) 5´CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC3´ 66.4
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