Glasgow/Wetlab/Orders
From 2007.igem.org
(Difference between revisions)
(→Designed primers) |
(→Designed primers) |
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|59.8 | |59.8 | ||
|- | |- | ||
| - | |Pu_Prefix_1( | + | |Pu_Prefix_1(After XylR) |
|5´GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG3´ | |5´GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG3´ | ||
|65.0 | |65.0 | ||
|- | |- | ||
| - | |Pu_Suffix_1( | + | |Pu_Suffix_1(After XylR) |
|5´CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG3´ | |5´CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG3´ | ||
|65.1 | |65.1 | ||
Revision as of 10:25, 16 August 2007
Emma's primers
| Name | Sequence | Tm |
|---|---|---|
| *(***)-Up | 5' *** C 3' | 65.6 |
| *(***)-Low | 5' *** 3' | 64.8 |
| *Bbp_(***)_Up | 5' *** 3' | 63.6 |
| *Bbs_(***)_Low | 5' *** 3' | 64.2 |
| Bbp_Gene | 5' gaa ttc gcg gcc gct tct ag 3' | 65.7 |
| Bbs | 5' ctg cag cgg ccg cta cta gta 3' | 65.3 |
| *Methyl_1 | 5' *** 3' | 66.3 |
| *Methyl_2 | 5' *** 3' | 66.2 |
| *Bbp_Methyl_1 | 5' *** 3' | 63.9 |
| *Bbs_Methyl_2 | 5' *** 3' | 66.9 |
| (***)_M_for_1 | 5' *** 3' | 65.4 |
| (***)_M_rev_1 | 5' *** 3' | 60.3 |
| Bbp_(***)_M_for_1 | 5' *** 3' | 64.5 |
| Bbs_(***)_M_rev_1 | 5' *** 3' | 60.3 |
| *Oxy_1 | 5' *** 3' | 60.2 |
| *Oxy_2 | 5' *** 3' | 59.2 |
| *Bbp_Oxy_1 | 5' *** 3' | 65.1 |
| *Bbs_Oxy_2 | 5' *** 3' | 66.9 |
| (***)_S_for_1 | 5' *** 3' | 71.2 |
| (***)_S_rev_1 | 5' *** 3' | 60.2 |
| Bbp_(***)_S_for_1 | 5' *** 3' | 70.9 |
| Bbs_(***)_S_rev_1 | 5' *** 3' | 63.3 |
| (***)_H_up | 5' *** 3' | 69.9 |
| (***)_H_low | 5' *** 3' | 70.6 |
| Bbp_(***)_Up | 5' *** 3' | 63.3 |
| Bbs_(***)_Low | 5' *** 3' | 57.7 |
Designed primers
| Name | Sequence | Tm |
|---|---|---|
| DmpR_SDM_PstI_1_for | 5´CTGGAGCAACAGCATGCGCTGTTC3´ | 65.6 |
| DmpR_SDM_PstI_1_rev | 5´GCTCCAGTTTTCAGCGATGGCC3´ | 64.8 |
| DmpR_SDM_PstI_2_for | 5´CTGGAGGAAGTGCTCAACCAGCAG3´ | 66.3 |
| DmpR_SDM_PstI_2_rev | 5´CCTCCAGCGCTTCCACCAG3´ | 66.2 |
| DmpR_Start_Prefix_1_for | 5´GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG3´ | 60.2 |
| DmpR_End_Suffix_1_rev | 5´CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC3´ | 59.2 |
| ***M_SDM_PstI_1_for | 5´***3´ | 69.9 |
| ***M_SDM_PstI_1_rev | 5´***3´ | 70.6 |
| ***S_SDM_PstI_1_for | 5´***3´ | 65.7 |
| ***S_SDM_PstI_1_rev | 5´***3´ | 65.3 |
| ***S_SDM_PstI_2_for | 5´***3´ | 63.6 |
| ***S_SDM_PstI_2_rev | 5´***3´ | 63.9 |
| ***C_SDM_PstI_1_for | 5´***3´ | 65.1 |
| ***C_SDM_PstI_1_rev | 5´***3´ | 64.2 |
| ***F_SDM_PstI_1_for | 5´***3´ | 66.9 |
| ***F_SDM_PstI_1_rev | 5´***3´ | 66.9 |
| ***B_SDM_EcoRI_1_for | 5´***3´ | 65.4 |
| ***B_SDM_EcoRI_1_rev | 5´***3´ | 64.5 |
| ***E_SDM_EcoRI_1_for | 5´***3´ | 60.3 |
| ***E_SDM_EcoRI_1_rev | 5´***3´ | 60.3 |
| ***E_SDM_PstI_1_for | 5´***3´ | 71.2 |
| ***E_SDM_PstI_1_rev | 5´***3´ | 70.9 |
| XylR_SDM_PstI_1_for | 5´GAGCTCCAGACCCAGGTTG3´ | 63.3 |
| XylR_SDM_PstI_1_rev | 5´GTCTGGAGCTCGTAGCGCTC3´ | 63.3 |
| XylR_Prefix_1 | 5´GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG3´ | 57.7 |
| XylR_Suffix_1 | 5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC3´ | 59.8 |
| Pu_Prefix_1(After XylR) | 5´GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG3´ | 65.0 |
| Pu_Suffix_1(After XylR) | 5´CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG3´ | 65.1 |
| Pu_Prefix_2(Emma´s sequence) | 5´GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC3´ | 66.0 |
| Pu_Suffix_2(Emma´s sequence) | 5´CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC3´ | 66.4 |
| DntR_Prefix_1 | 5´GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG3´ | 61.2 |
| DntR_Suffix_1 | 5´TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT3´ | 55.2 |
| DntR_Suffix_2 | 5´CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG3´ | 55.3 |
| Pr_Prefix_1 | 5´GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG3´ | 58.8 |
| Pr_Suffix_1 | 5´CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG3´ | 57.3 |
| p1010_for | 5´GCTGTGTATAAGGGAGCCTGA3´ | 59.4 |
| p1010_rev | 5´TGACAGTTGACAGCGACAGC3´ | 61.3 |
| bbp_XylR_for_2 | 5´GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC3´ | 55.2 |
| bbs_XylR_rev_2 | 5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG3´ | 61.8 |
| bbp_Pr_for_2 | 5´GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG3´ | 60.8 |
| bbs_Pr_rev_2 | 5´CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC3´ | 61.3 |
| bbp_Pu_for_2 | 5´GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC3´ | 64.3 |
| bbs_Pu_rev_2 | 5´CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG3´ | 69.9 |
| bbp_***D_for | 5´***3´ | 61.0 |
| bbs_***D_for | 5´***3´ | 61.4 |
| bbp_***A_for | 5´***3´ | 63.1 |
| bbs_***G_rev | 5´***3´ | 61.9 |
