Glasgow/Wetlab/Orders

From 2007.igem.org

(Difference between revisions)
(→Designed primers)
(→Emma's primers)
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!Tm
!Tm
|-
|-
-
|*(***)-Up
+
|* ***Up
-
|5' *** C 3'
+
|5´ *** 3´
|65.6
|65.6
|-
|-
-
|*(***)-Low
+
|* ***Low
-
|5' *** 3'
+
|5´ *** 3´
|64.8
|64.8
|-
|-
-
|*Bbp_(***)_Up
+
|*Bbp_***Up
-
|5' *** 3'
+
|5´ *** 3´
|63.6
|63.6
|-
|-
-
|*Bbs_(***)_Low
+
|*Bbs_***Low
-
|5' *** 3'
+
|5´ *** 3´
|64.2
|64.2
|-
|-
|Bbp_Gene
|Bbp_Gene
-
|5' gaa ttc gcg gcc gct tct ag 3'
+
|5´ gaattcgcggccgcttctag 3´
|65.7
|65.7
|-
|-
|Bbs
|Bbs
-
|5' ctg cag cgg ccg cta cta gta 3'
+
|5´ ctgcagcggccgctactagta 3´
|65.3
|65.3
|-
|-
|*Methyl_1
|*Methyl_1
-
|5' *** 3'
+
|5´ *** 3´
|66.3
|66.3
|-
|-
|*Methyl_2
|*Methyl_2
-
|5' *** 3'
+
|5´ *** 3´
|66.2
|66.2
|-
|-
|*Bbp_Methyl_1
|*Bbp_Methyl_1
-
|5' *** 3'
+
|5´ *** 3´
|63.9
|63.9
|-
|-
|*Bbs_Methyl_2
|*Bbs_Methyl_2
-
|5' *** 3'
+
|5´ *** 3´
|66.9
|66.9
|-
|-
-
|(***)_M_for_1
+
|***M_for_1
-
|5' *** 3'
+
|5´ *** 3´
|65.4
|65.4
|-
|-
-
|(***)_M_rev_1
+
|***M_rev_1
-
|5' *** 3'
+
|5´ *** 3´
|60.3
|60.3
|-
|-
-
|Bbp_(***)_M_for_1
+
|Bbp_***M_for_1
-
|5' *** 3'
+
|5´ *** 3´
|64.5
|64.5
|-
|-
-
|Bbs_(***)_M_rev_1
+
|Bbs_***M_rev_1
-
|5' *** 3'
+
|5´ *** 3´
|60.3
|60.3
|-
|-
|*Oxy_1
|*Oxy_1
-
|5' *** 3'
+
|5´ *** 3´
|60.2
|60.2
|-
|-
|*Oxy_2
|*Oxy_2
-
|5' *** 3'
+
|5´ *** 3´
|59.2
|59.2
|-
|-
|*Bbp_Oxy_1
|*Bbp_Oxy_1
-
|5' *** 3'
+
|5´ *** 3´
|65.1
|65.1
|-
|-
|*Bbs_Oxy_2
|*Bbs_Oxy_2
-
|5' *** 3'
+
|5´ *** 3´
|66.9
|66.9
|-
|-
-
|(***)_S_for_1
+
|***S_for_1
-
|5' *** 3'
+
|5´ *** 3´
|71.2
|71.2
|-
|-
-
|(***)_S_rev_1
+
|***S_rev_1
-
|5' *** 3'
+
|5´ *** 3´
|60.2
|60.2
|-
|-
-
|Bbp_(***)_S_for_1
+
|Bbp_***S_for_1
-
|5' *** 3'
+
|5´ *** 3´
|70.9
|70.9
|-
|-
-
|Bbs_(***)_S_rev_1
+
|Bbs_***S_rev_1
-
|5' *** 3'
+
|5´ *** 3´
|63.3
|63.3
|-
|-
-
|(***)_H_up
+
|***H_up
-
|5' *** 3'
+
|5´ *** 3´
|69.9
|69.9
|-
|-
-
|(***)_H_low
+
|***H_low
-
|5' *** 3'
+
|5´ *** 3´
|70.6
|70.6
|-
|-
-
|Bbp_(***)_Up
+
|Bbp_***Up
-
|5' *** 3'
+
|5´ *** 3´
|63.3
|63.3
|-
|-
-
|Bbs_(***)_Low
+
|Bbs_***Low
-
|5' *** 3'
+
|5´ *** 3´
|57.7
|57.7
|}
|}

Revision as of 10:30, 16 August 2007

Glasgow Main Page

Emma's primers

Name Sequence Tm
* ***Up 5´ *** 3´ 65.6
* ***Low 5´ *** 3´ 64.8
*Bbp_***Up 5´ *** 3´ 63.6
*Bbs_***Low 5´ *** 3´ 64.2
Bbp_Gene 5´ gaattcgcggccgcttctag 3´ 65.7
Bbs 5´ ctgcagcggccgctactagta 3´ 65.3
*Methyl_1 5´ *** 3´ 66.3
*Methyl_2 5´ *** 3´ 66.2
*Bbp_Methyl_1 5´ *** 3´ 63.9
*Bbs_Methyl_2 5´ *** 3´ 66.9
***M_for_1 5´ *** 3´ 65.4
***M_rev_1 5´ *** 3´ 60.3
Bbp_***M_for_1 5´ *** 3´ 64.5
Bbs_***M_rev_1 5´ *** 3´ 60.3
*Oxy_1 5´ *** 3´ 60.2
*Oxy_2 5´ *** 3´ 59.2
*Bbp_Oxy_1 5´ *** 3´ 65.1
*Bbs_Oxy_2 5´ *** 3´ 66.9
***S_for_1 5´ *** 3´ 71.2
***S_rev_1 5´ *** 3´ 60.2
Bbp_***S_for_1 5´ *** 3´ 70.9
Bbs_***S_rev_1 5´ *** 3´ 63.3
***H_up 5´ *** 3´ 69.9
***H_low 5´ *** 3´ 70.6
Bbp_***Up 5´ *** 3´ 63.3
Bbs_***Low 5´ *** 3´ 57.7

Designed primers

Name Sequence Tm
DmpR_SDM_PstI_1_for 5´CTGGAGCAACAGCATGCGCTGTTC3´ 65.6
DmpR_SDM_PstI_1_rev 5´GCTCCAGTTTTCAGCGATGGCC3´ 64.8
DmpR_SDM_PstI_2_for 5´CTGGAGGAAGTGCTCAACCAGCAG3´ 66.3
DmpR_SDM_PstI_2_rev 5´CCTCCAGCGCTTCCACCAG3´ 66.2
DmpR_Start_Prefix_1_for 5´GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG3´ 60.2
DmpR_End_Suffix_1_rev 5´CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC3´ 59.2
***M_SDM_PstI_1_for 5´***3´ 69.9
***M_SDM_PstI_1_rev 5´***3´ 70.6
***S_SDM_PstI_1_for 5´***3´ 65.7
***S_SDM_PstI_1_rev 5´***3´ 65.3
***S_SDM_PstI_2_for 5´***3´ 63.6
***S_SDM_PstI_2_rev 5´***3´ 63.9
***C_SDM_PstI_1_for 5´***3´ 65.1
***C_SDM_PstI_1_rev 5´***3´ 64.2
***F_SDM_PstI_1_for 5´***3´ 66.9
***F_SDM_PstI_1_rev 5´***3´ 66.9
***B_SDM_EcoRI_1_for 5´***3´ 65.4
***B_SDM_EcoRI_1_rev 5´***3´ 64.5
***E_SDM_EcoRI_1_for 5´***3´ 60.3
***E_SDM_EcoRI_1_rev 5´***3´ 60.3
***E_SDM_PstI_1_for 5´***3´ 71.2
***E_SDM_PstI_1_rev 5´***3´ 70.9
XylR_SDM_PstI_1_for 5´GAGCTCCAGACCCAGGTTG3´ 63.3
XylR_SDM_PstI_1_rev 5´GTCTGGAGCTCGTAGCGCTC3´ 63.3
XylR_Prefix_1 5´GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG3´ 57.7
XylR_Suffix_1 5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC3´ 59.8
Pu_Prefix_1(After XylR) 5´GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG3´ 65.0
Pu_Suffix_1(After XylR) 5´CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG3´ 65.1
Pu_Prefix_2(Emma´s sequence) 5´GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC3´ 66.0
Pu_Suffix_2(Emma´s sequence) 5´CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC3´ 66.4
DntR_Prefix_1 5´GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG3´ 61.2
DntR_Suffix_1 5´TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT3´ 55.2
DntR_Suffix_2 5´CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG3´ 55.3
Pr_Prefix_1 5´GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG3´ 58.8
Pr_Suffix_1 5´CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG3´ 57.3
p1010_for 5´GCTGTGTATAAGGGAGCCTGA3´ 59.4
p1010_rev 5´TGACAGTTGACAGCGACAGC3´ 61.3
bbp_XylR_for_2 5´GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC3´ 55.2
bbs_XylR_rev_2 5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG3´ 61.8
bbp_Pr_for_2 5´GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG3´ 60.8
bbs_Pr_rev_2 5´CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC3´ 61.3
bbp_Pu_for_2 5´GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC3´ 64.3
bbs_Pu_rev_2 5´CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG3´ 69.9
bbp_***D_for 5´***3´ 61.0
bbs_***D_for 5´***3´ 61.4
bbp_***A_for 5´***3´ 63.1
bbs_***G_rev 5´***3´ 61.9
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