Glasgow/Wetlab/Orders

From 2007.igem.org

(Difference between revisions)
(→Emma's primers)
(→Designed primers)
Line 120: Line 120:
|-
|-
|DmpR_SDM_PstI_1_for
|DmpR_SDM_PstI_1_for
-
|5´CTGGAGCAACAGCATGCGCTGTTC3´
+
|5´ CTGGAGCAACAGCATGCGCTGTTC 3´
|65.6
|65.6
|-
|-
|DmpR_SDM_PstI_1_rev
|DmpR_SDM_PstI_1_rev
-
|5´GCTCCAGTTTTCAGCGATGGCC3´
+
|5´ GCTCCAGTTTTCAGCGATGGCC 3´
|64.8
|64.8
|-
|-
|DmpR_SDM_PstI_2_for
|DmpR_SDM_PstI_2_for
-
|5´CTGGAGGAAGTGCTCAACCAGCAG3´
+
|5´ CTGGAGGAAGTGCTCAACCAGCAG 3´
|66.3
|66.3
|-
|-
|DmpR_SDM_PstI_2_rev
|DmpR_SDM_PstI_2_rev
-
|5´CCTCCAGCGCTTCCACCAG3´
+
|5´ CCTCCAGCGCTTCCACCAG 3´
|66.2
|66.2
|-
|-
|DmpR_Start_Prefix_1_for
|DmpR_Start_Prefix_1_for
-
|5´GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG3´
+
|5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´
|60.2
|60.2
|-
|-
|DmpR_End_Suffix_1_rev
|DmpR_End_Suffix_1_rev
-
|5´CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC3´
+
|5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´
|59.2
|59.2
|-
|-
|***M_SDM_PstI_1_for
|***M_SDM_PstI_1_for
-
|5´***3´
+
|5´ *** 3´
|69.9
|69.9
|-
|-
|***M_SDM_PstI_1_rev
|***M_SDM_PstI_1_rev
-
|5´***3´
+
|5´ *** 3´
|70.6
|70.6
|-
|-
|***S_SDM_PstI_1_for
|***S_SDM_PstI_1_for
-
|5´***3´
+
|5´ *** 3´
|65.7
|65.7
|-
|-
|***S_SDM_PstI_1_rev
|***S_SDM_PstI_1_rev
-
|5´***3´
+
|5´ *** 3´
|65.3
|65.3
|-
|-
|***S_SDM_PstI_2_for
|***S_SDM_PstI_2_for
-
|5´***3´
+
|5´ *** 3´
|63.6
|63.6
|-
|-
|***S_SDM_PstI_2_rev
|***S_SDM_PstI_2_rev
-
|5´***3´
+
|5´ *** 3´
|63.9
|63.9
|-
|-
|***C_SDM_PstI_1_for
|***C_SDM_PstI_1_for
-
|5´***3´
+
|5´ *** 3´
|65.1
|65.1
|-
|-
|***C_SDM_PstI_1_rev
|***C_SDM_PstI_1_rev
-
|5´***3´
+
|5´ *** 3´
|64.2
|64.2
|-
|-
|***F_SDM_PstI_1_for
|***F_SDM_PstI_1_for
-
|5´***3´
+
|5´ *** 3´
|66.9
|66.9
|-
|-
|***F_SDM_PstI_1_rev
|***F_SDM_PstI_1_rev
-
|5´***3´
+
|5´ *** 3´
|66.9
|66.9
|-
|-
|***B_SDM_EcoRI_1_for
|***B_SDM_EcoRI_1_for
-
|5´***3´
+
|5´ *** 3´
|65.4
|65.4
|-
|-
|***B_SDM_EcoRI_1_rev
|***B_SDM_EcoRI_1_rev
-
|5´***3´
+
|5´ *** 3´
|64.5
|64.5
|-
|-
|***E_SDM_EcoRI_1_for
|***E_SDM_EcoRI_1_for
-
|5´***3´
+
|5´ *** 3´
|60.3
|60.3
|-
|-
|***E_SDM_EcoRI_1_rev
|***E_SDM_EcoRI_1_rev
-
|5´***3´
+
|5´ *** 3´
|60.3
|60.3
|-
|-
|***E_SDM_PstI_1_for
|***E_SDM_PstI_1_for
-
|5´***3´
+
|5´ *** 3´
|71.2
|71.2
|-
|-
|***E_SDM_PstI_1_rev
|***E_SDM_PstI_1_rev
-
|5´***3´
+
|5´ *** 3´
|70.9
|70.9
|-
|-
|XylR_SDM_PstI_1_for
|XylR_SDM_PstI_1_for
-
|5´GAGCTCCAGACCCAGGTTG3´
+
|5´ GAGCTCCAGACCCAGGTTG 3´
|63.3
|63.3
|-
|-
|XylR_SDM_PstI_1_rev
|XylR_SDM_PstI_1_rev
-
|5´GTCTGGAGCTCGTAGCGCTC3´
+
|5´ GTCTGGAGCTCGTAGCGCTC 3´
|63.3
|63.3
|-
|-
|XylR_Prefix_1
|XylR_Prefix_1
-
|5´GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG3´
+
|5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´
|57.7
|57.7
|-
|-
|XylR_Suffix_1
|XylR_Suffix_1
-
|5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC3´
+
|5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´
|59.8
|59.8
|-
|-
|Pu_Prefix_1(After XylR)
|Pu_Prefix_1(After XylR)
-
|5´GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG3´
+
|5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´
|65.0
|65.0
|-
|-
|Pu_Suffix_1(After XylR)
|Pu_Suffix_1(After XylR)
-
|5´CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG3´
+
|5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´
|65.1
|65.1
|-
|-
|Pu_Prefix_2(Emma´s sequence)
|Pu_Prefix_2(Emma´s sequence)
-
|5´GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC3´
+
|5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´
|66.0
|66.0
|-
|-
|Pu_Suffix_2(Emma´s sequence)
|Pu_Suffix_2(Emma´s sequence)
-
|5´CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC3´
+
|5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´
|66.4
|66.4
|-
|-
|DntR_Prefix_1
|DntR_Prefix_1
-
|5´GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG3´
+
|5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´
|61.2
|61.2
|-
|-
|DntR_Suffix_1
|DntR_Suffix_1
-
|5´TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT3´
+
|5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´
|55.2
|55.2
|-
|-
|DntR_Suffix_2
|DntR_Suffix_2
-
|5´CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG3´
+
|5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´
|55.3
|55.3
|-
|-
|Pr_Prefix_1
|Pr_Prefix_1
-
|5´GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG3´
+
|5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´
|58.8
|58.8
|-
|-
|Pr_Suffix_1
|Pr_Suffix_1
-
|5´CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG3´
+
|5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´
|57.3
|57.3
|-
|-
|p1010_for
|p1010_for
-
|5´GCTGTGTATAAGGGAGCCTGA3´
+
|5´ GCTGTGTATAAGGGAGCCTGA 3´
|59.4
|59.4
|-
|-
|p1010_rev
|p1010_rev
-
|5´TGACAGTTGACAGCGACAGC3´
+
|5´ TGACAGTTGACAGCGACAGC 3´
|61.3
|61.3
|-
|-
|bbp_XylR_for_2
|bbp_XylR_for_2
-
|5´GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC3´
+
|5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´
|55.2
|55.2
|-
|-
|bbs_XylR_rev_2
|bbs_XylR_rev_2
-
|5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG3´
+
|5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´
|61.8
|61.8
|-
|-
|bbp_Pr_for_2
|bbp_Pr_for_2
-
|5´GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG3´
+
|5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´
|60.8
|60.8
|-
|-
|bbs_Pr_rev_2
|bbs_Pr_rev_2
-
|5´CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC3´
+
|5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´
|61.3
|61.3
|-
|-
|bbp_Pu_for_2
|bbp_Pu_for_2
-
|5´GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC3´
+
|5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´
|64.3
|64.3
|-
|-
|bbs_Pu_rev_2
|bbs_Pu_rev_2
-
|5´CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG3´
+
|5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´
|69.9
|69.9
|-
|-
|bbp_***D_for
|bbp_***D_for
-
|5´***3´
+
|5´ *** 3´
|61.0
|61.0
|-
|-
|bbs_***D_for
|bbs_***D_for
-
|5´***3´
+
|5´ *** 3´
|61.4
|61.4
|-
|-
|bbp_***A_for
|bbp_***A_for
-
|5´***3´
+
|5´ *** 3´
|63.1
|63.1
|-
|-
|bbs_***G_rev
|bbs_***G_rev
-
|5´***3´
+
|5´ *** 3´
|61.9
|61.9
|}
|}

Revision as of 10:32, 16 August 2007

Glasgow Main Page

Emma's primers

Name Sequence Tm
* ***Up 5´ *** 3´ 65.6
* ***Low 5´ *** 3´ 64.8
*Bbp_***Up 5´ *** 3´ 63.6
*Bbs_***Low 5´ *** 3´ 64.2
Bbp_Gene 5´ gaattcgcggccgcttctag 3´ 65.7
Bbs 5´ ctgcagcggccgctactagta 3´ 65.3
*Methyl_1 5´ *** 3´ 66.3
*Methyl_2 5´ *** 3´ 66.2
*Bbp_Methyl_1 5´ *** 3´ 63.9
*Bbs_Methyl_2 5´ *** 3´ 66.9
***M_for_1 5´ *** 3´ 65.4
***M_rev_1 5´ *** 3´ 60.3
Bbp_***M_for_1 5´ *** 3´ 64.5
Bbs_***M_rev_1 5´ *** 3´ 60.3
*Oxy_1 5´ *** 3´ 60.2
*Oxy_2 5´ *** 3´ 59.2
*Bbp_Oxy_1 5´ *** 3´ 65.1
*Bbs_Oxy_2 5´ *** 3´ 66.9
***S_for_1 5´ *** 3´ 71.2
***S_rev_1 5´ *** 3´ 60.2
Bbp_***S_for_1 5´ *** 3´ 70.9
Bbs_***S_rev_1 5´ *** 3´ 63.3
***H_up 5´ *** 3´ 69.9
***H_low 5´ *** 3´ 70.6
Bbp_***Up 5´ *** 3´ 63.3
Bbs_***Low 5´ *** 3´ 57.7

Designed primers

Name Sequence Tm
DmpR_SDM_PstI_1_for 5´ CTGGAGCAACAGCATGCGCTGTTC 3´ 65.6
DmpR_SDM_PstI_1_rev 5´ GCTCCAGTTTTCAGCGATGGCC 3´ 64.8
DmpR_SDM_PstI_2_for 5´ CTGGAGGAAGTGCTCAACCAGCAG 3´ 66.3
DmpR_SDM_PstI_2_rev 5´ CCTCCAGCGCTTCCACCAG 3´ 66.2
DmpR_Start_Prefix_1_for 5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´ 60.2
DmpR_End_Suffix_1_rev 5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´ 59.2
***M_SDM_PstI_1_for 5´ *** 3´ 69.9
***M_SDM_PstI_1_rev 5´ *** 3´ 70.6
***S_SDM_PstI_1_for 5´ *** 3´ 65.7
***S_SDM_PstI_1_rev 5´ *** 3´ 65.3
***S_SDM_PstI_2_for 5´ *** 3´ 63.6
***S_SDM_PstI_2_rev 5´ *** 3´ 63.9
***C_SDM_PstI_1_for 5´ *** 3´ 65.1
***C_SDM_PstI_1_rev 5´ *** 3´ 64.2
***F_SDM_PstI_1_for 5´ *** 3´ 66.9
***F_SDM_PstI_1_rev 5´ *** 3´ 66.9
***B_SDM_EcoRI_1_for 5´ *** 3´ 65.4
***B_SDM_EcoRI_1_rev 5´ *** 3´ 64.5
***E_SDM_EcoRI_1_for 5´ *** 3´ 60.3
***E_SDM_EcoRI_1_rev 5´ *** 3´ 60.3
***E_SDM_PstI_1_for 5´ *** 3´ 71.2
***E_SDM_PstI_1_rev 5´ *** 3´ 70.9
XylR_SDM_PstI_1_for 5´ GAGCTCCAGACCCAGGTTG 3´ 63.3
XylR_SDM_PstI_1_rev 5´ GTCTGGAGCTCGTAGCGCTC 3´ 63.3
XylR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´ 57.7
XylR_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´ 59.8
Pu_Prefix_1(After XylR) 5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´ 65.0
Pu_Suffix_1(After XylR) 5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´ 65.1
Pu_Prefix_2(Emma´s sequence) 5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´ 66.0
Pu_Suffix_2(Emma´s sequence) 5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´ 66.4
DntR_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´ 61.2
DntR_Suffix_1 5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´ 55.2
DntR_Suffix_2 5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´ 55.3
Pr_Prefix_1 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´ 58.8
Pr_Suffix_1 5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´ 57.3
p1010_for 5´ GCTGTGTATAAGGGAGCCTGA 3´ 59.4
p1010_rev 5´ TGACAGTTGACAGCGACAGC 3´ 61.3
bbp_XylR_for_2 5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ 55.2
bbs_XylR_rev_2 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ 61.8
bbp_Pr_for_2 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ 60.8
bbs_Pr_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ 61.3
bbp_Pu_for_2 5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ 64.3
bbs_Pu_rev_2 5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ 69.9
bbp_***D_for 5´ *** 3´ 61.0
bbs_***D_for 5´ *** 3´ 61.4
bbp_***A_for 5´ *** 3´ 63.1
bbs_***G_rev 5´ *** 3´ 61.9
Retrieved from "http://2007.igem.org/wiki/index.php/Glasgow/Wetlab/Orders"