Glasgow/Wetlab

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<br>Primer design for death gene.
<br>Primer design for death gene.
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!width="180" |[https://2007.igem.org/Glasgow/Wetlab/Week5 https://static.igem.org/mediawiki/2007/4/4f/Week_5.png] ||  
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<br>Suspected amplification of 7 gene operon.
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<br>*m and *s confirmed as present in TOPO vectors.
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<br>Confirmed presence of death gene plasmid in some vectors.
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<br>Miller assay performed with DntR system.
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<br>Yeast cells.
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Revision as of 19:54, 19 August 2007

Glasgow Main Page


Click on a week number for more detailed lab book...

https://static.igem.org/mediawiki/2007/c/ce/Week_1.png First selection of biobricks transformed.
Preparation of stocks.
https://static.igem.org/mediawiki/2007/2/20/Week_2.png


Change of plan because DmpR failed to grow.
Researched XylR and DntR sensor systems.
Designed primers for DmpR, DntR, XylR and associated promoters.

https://static.igem.org/mediawiki/2007/7/74/Week_3.png


BioBricks tested by digestion and PCR.
PCR with new primers associated with DntR and XylR.

https://static.igem.org/mediawiki/2007/3/34/Week_4.png


*m and *s cloned into TOPO vectors.
Primer design for 7 gene operon.
DntR cloned into TOPO vector.
Primer design for XylR and associated genes.
Death gene cloned into TOPO vector.
Primer design for death gene.

https://static.igem.org/mediawiki/2007/4/4f/Week_5.png


Suspected amplification of 7 gene operon.
*m and *s confirmed as present in TOPO vectors.
Confirmed presence of death gene plasmid in some vectors.
Miller assay performed with DntR system.
Yeast cells.

https://static.igem.org/mediawiki/2007/7/70/Week_6.png Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
https://static.igem.org/mediawiki/2007/c/c0/Week_7.png Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
https://static.igem.org/mediawiki/2007/9/9b/Week_8.png Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
https://static.igem.org/mediawiki/2007/a/a1/Week_9.png Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
https://static.igem.org/mediawiki/2007/8/88/Week_10.png Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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