Glasgow/Wetlab/Week8

From 2007.igem.org

(Difference between revisions)
(→Tuesday 21st August 2007)
(→Tuesday 21st August 2007)
Line 23: Line 23:
=== Tuesday 21st August 2007 ===
=== Tuesday 21st August 2007 ===
-
#Maija digested the construction vectors 3/20G and 4/6B, and the 7 gene operon with SpeI and XbaI ([[Glasgow/Wetlab/Protocols#Protocol 7: Restriction Digests|Protocol 7]]) with the intention of then ligating the 7 gene operon into the construction vectors.  The 7 gene operon DNA (taken from sample 2 of the PCR which was gel extracted 13/08/07) did not appear on the gel.
+
<ol>
 +
<li>
 +
Maija digested the construction vectors 3/20G and 4/6B, and the 7 gene operon with SpeI and XbaI ([[Glasgow/Wetlab/Protocols#Protocol 7: Restriction Digests|Protocol 7]]) with the intention of then ligating the 7 gene operon into the construction vectors.  The 7 gene operon DNA (taken from sample 2 of the PCR which was gel extracted 13/08/07) did not appear on the gel.
 +
{| border="1"
 +
!Label
 +
!Description
 +
!Enzymes
 +
!Expected size
 +
!Result
 +
|-
 +
|3/20G
 +
|Construction vector
 +
|SpeI, XbaI
 +
|2071bp
 +
|2071bp
 +
|-
 +
|4/6B
 +
|Construction vector
 +
|SpeI, XbaI
 +
|2928bp
 +
|2928bp
 +
|-
 +
|
=== Wednesday 22nd August 2007 ===
=== Wednesday 22nd August 2007 ===

Revision as of 17:39, 21 August 2007

Back To Main Page | Back To Wetlab Log

Contents

Week 8

Monday 20th August 2007

  1. Chrsitine did more minipreps for colonies suspected of containing the 7 gene operon. When these minipreps were run on a gel there was no evidence of an insert.


  1. These
  2. are
    1. numbered
  3. sections



This is a table
Look at the code to see the template.

Tuesday 21st August 2007

  1. Maija digested the construction vectors 3/20G and 4/6B, and the 7 gene operon with SpeI and XbaI (Protocol 7) with the intention of then ligating the 7 gene operon into the construction vectors. The 7 gene operon DNA (taken from sample 2 of the PCR which was gel extracted 13/08/07) did not appear on the gel.
    Label Description Enzymes Expected size Result
    3/20G Construction vector SpeI, XbaI 2071bp 2071bp
    4/6B Construction vector SpeI, XbaI 2928bp 2928bp

    Wednesday 22nd August 2007

    Thursday 23rd August 2007

    === Friday 24th August 2007 ===
    Retrieved from "http://2007.igem.org/wiki/index.php/Glasgow/Wetlab/Week8"