Glasgow/Wetlab

From 2007.igem.org

(Difference between revisions)
(WIP)
(Re-organised layout)
Line 2: Line 2:
----
----
{|cellspacing="6px" cellpadding="16" border="0" width="100%"
{|cellspacing="6px" cellpadding="16" border="0" width="100%"
-
|-
+
|- align=center
-
|[[Glasgow/Wetlab/Protocols|PROTOCOLS]]
+
|[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=#99CCFF size=5><b>Protocols</b></font>]
-
|[[Glasgow/Wetlab/References|REFERENCES]]
+
|[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>]
-
|[[Glasgow/Wetlab/Resources|RESOURCES]]
+
|[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=#99CCFF size=5><b>Resources</b></font>]
-
|[[Glasgow/Wetlab/Orders|ORDERS]]
+
|[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Orders</b></font>]
-
|[[Glasgow/Wetlab/Biobricks|BIOBRICKS UTILISED]]
+
|[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#99CCFF size=5><b>Biobricks<br>Used</b></font>]
-
|[[Glasgow/Wetlab/Gels|GELS]]
+
|[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>]
-
 
+
|}
|}
 +
----
----
-
 
''Click on a week number for more detailed lab book...''
''Click on a week number for more detailed lab book...''
Line 19: Line 18:
{|cellspacing="6px" cellpadding="16" border="0" width="100%"
{|cellspacing="6px" cellpadding="16" border="0" width="100%"
|- align="center"
|- align="center"
-
!width="50%" | [https://2007.igem.org/Glasgow/Wetlab/Week1 https://static.igem.org/mediawiki/2007/c/ce/Week_1.png] <br>  
+
!width="50%" | [https://2007.igem.org/Glasgow/Wetlab/Week1 <font face=georgia color=#3366CC size=7><b>Week 1</b></font>] <br>  
-
*First selection of biobricks transformed.
+
-
*Preparation of stocks.
+
-
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week2 https://static.igem.org/mediawiki/2007/2/20/Week_2.png] <br>  
+
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week2 <font face=georgia color=#3366CC size=7><b>Week 2</b></font>] <br>  
-
*Change of plan because DmpR failed to grow.  
+
 
-
*Researched XylR and DntR sensor systems.
+
|- valign=top
-
*Designed primers for DmpR, DntR, XylR and associated promoters.
+
|
 +
*'''''First selection of biobricks transformed.'''
 +
*'''''Preparation of stocks.'''
 +
|
 +
*'''''Change of plan because DmpR failed to grow. '''
 +
*'''''Researched XylR and DntR sensor systems.'''
 +
*'''''Designed primers for DmpR, DntR, XylR and associated promoters.'''
|- align="center"
|- align="center"
-
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week3 https://static.igem.org/mediawiki/2007/7/74/Week_3.png] <br>  
+
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week3 <font face=georgia color=#99CCFF size=7><b>Week 3</b></font>] <br>  
-
*BioBricks tested by digestion and PCR.
+
-
*PCR with new primers associated with DntR and XylR.
+
-
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week4 https://static.igem.org/mediawiki/2007/3/34/Week_4.png] <br>  
+
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week4 <font face=georgia color=#99CCFF size=7><b>Week 4</b></font>] <br>  
-
**m and *s cloned into TOPO vectors.
+
-
*Primer design for 7 gene operon.
+
-
*DntR cloned into TOPO vector.
+
-
*Primer design for XylR and associated genes.
+
-
*Death gene cloned into TOPO vector.
+
-
*Primer design for death gene.
+
-
|- align="left"
+
|- valign=top
-
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week5 https://static.igem.org/mediawiki/2007/4/4f/Week_5.png] <br>
+
|
-
*Suspected amplification of 7 gene operon.
+
*'''BioBricks tested by digestion and PCR.'''
-
**m and *s confirmed as present in TOPO vectors.
+
*'''PCR with new primers associated with DntR and XylR.'''
-
*Confirmed presence of death gene plasmid in some vectors.
+
|
-
*Miller assay performed with DntR system.
+
* '''*m and *s cloned into TOPO vectors.'''
-
*Yeast cells.
+
*'''Primer design for 7 gene operon.'''
 +
*'''DntR cloned into TOPO vector.'''
 +
*'''Primer design for XylR and associated genes.'''
 +
*'''Death gene cloned into TOPO vector.'''
 +
*'''Primer design for death gene.'''
-
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week6 https://static.igem.org/mediawiki/2007/7/70/Week_6.png] <br>
+
|- align="center"
 +
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week5 <font face=georgia color=#3366CC size=7><b>Week 5</b></font>] <br>
 +
 
 +
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week6 <font face=georgia color=#3366CC size=7><b>Week 6</b></font>] <br>
 +
 
 +
|- valign=top
 +
|
 +
*'''Suspected amplification of 7 gene operon.'''
 +
* '''*m and *s confirmed as present in TOPO vectors.'''
 +
*'''Confirmed presence of death gene plasmid in some vectors.'''
 +
*'''Miller assay performed with DntR system.'''
 +
*'''Yeast cells.'''
 +
|
*'''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
*'''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
-
|- align="left"
+
|- align="center"
-
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week7 https://static.igem.org/mediawiki/2007/c/c0/Week_7.png] <br>  
+
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week7 <font face=georgia color=#99CCFF size=7><b>Week 7</b></font>] <br>  
-
*Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
-
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week8 https://static.igem.org/mediawiki/2007/9/9b/Week_8.png] <br>  
+
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week8 <font face=georgia color=#99CCFF size=7><b>Week 8</b></font>] <br>  
 +
 
 +
|- valign=top
 +
|
 +
*'''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''
 +
|
*'''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
*'''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
-
|- align="left"
+
|- align="center"
-
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week9 https://static.igem.org/mediawiki/2007/a/a1/Week_9.png] <br>  
+
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week9 <font face=georgia color=#3366CC size=7><b>Week 9</b></font>] <br>  
-
*Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
-
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week10 https://static.igem.org/mediawiki/2007/8/88/Week_10.png] <br>  
+
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week10 <font face=georgia color=#3366CC size=7><b>Week 10</b></font>] <br>  
 +
 
 +
|- valign=top
 +
|
 +
*'''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''
 +
|
*'''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
*'''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
|}
|}
Line 70: Line 89:
{|cellspacing="6px" cellpadding="16" border="0" width="100%"
{|cellspacing="6px" cellpadding="16" border="0" width="100%"
-
|-
+
|- align=center
-
|[[Glasgow/Wetlab/Protocols|PROTOCOLS]]
+
|[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=#99CCFF size=5><b>Protocols</b></font>]
-
|[[Glasgow/Wetlab/References|REFERENCES]]
+
|[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>]
-
|[[Glasgow/Wetlab/Orders|ORDERS]]
+
|[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=#99CCFF size=5><b>Resources</b></font>]
-
|[[Glasgow/Wetlab/Biobricks|BIOBRICKS UTILISED]]
+
|[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Orders</b></font>]
-
|[[Glasgow/Wetlab/Gels|GELS]]
+
|[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#99CCFF size=5><b>Biobricks<br>Used</b></font>]
-
 
+
|[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>]
|}
|}

Revision as of 19:45, 24 August 2007

Glasgow Main Page

Protocols References Resources Orders Biobricks
Used 		Gels

Click on a week number for more detailed lab book...


Week 1
Week 2
  • First selection of biobricks transformed.
  • Preparation of stocks.
  • Change of plan because DmpR failed to grow.
  • Researched XylR and DntR sensor systems.
  • Designed primers for DmpR, DntR, XylR and associated promoters.
Week 3
Week 4
  • BioBricks tested by digestion and PCR.
  • PCR with new primers associated with DntR and XylR.
  • *m and *s cloned into TOPO vectors.
  • Primer design for 7 gene operon.
  • DntR cloned into TOPO vector.
  • Primer design for XylR and associated genes.
  • Death gene cloned into TOPO vector.
  • Primer design for death gene.
Week 5
Week 6
  • Suspected amplification of 7 gene operon.
  • *m and *s confirmed as present in TOPO vectors.
  • Confirmed presence of death gene plasmid in some vectors.
  • Miller assay performed with DntR system.
  • Yeast cells.
  • Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
Week 7
Week 8
  • Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
  • Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
Week 9
Week 10
  • Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
  • Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
Protocols References Resources Orders Biobricks
Used 		Gels
Retrieved from "http://2007.igem.org/wiki/index.php/Glasgow/Wetlab"