Week 2
From 2007.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
*We start plasmid amplification from IGEM 2007 plates. | *We start plasmid amplification from IGEM 2007 plates. | ||
| - | *We have some problems with Ampicillin solubility in water. We find that our Ampicillin | + | *We have some problems with Ampicillin solubility in water. We find that our Ampicillin is not soluble in water, so we try to re-suspend it in NaOH 1M with work concentration of 50 μg/ml. Since bacterial cells still grow on our plates, we try to change the antibiotic. |
| - | So, we use a water- soluble Ampicillin with | + | So, we use a water-soluble Ampicillin with work concentration of 100 μg/ml. We find that it finally worked. |
| - | *We also have some problems with Kanamycin. We first use it at | + | *We also have some problems with Kanamycin. We first use it at work concentration of 20 μg/ml, but it doesn’t work. Guys from the Boston University team have been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it works correctly. |
| - | *Preparation of [[Bologna_University/IPTG stocks preparation | IPTG stocks]] | + | *Preparation of 100 mM of[[Bologna_University/IPTG stocks preparation | IPTG stocks]]. |
[[Bologna | Back]] | [[Bologna | Back]] | ||
Revision as of 09:12, 5 September 2007
- 07/09/07 - 07/15/07
- We start plasmid amplification from IGEM 2007 plates.
- We have some problems with Ampicillin solubility in water. We find that our Ampicillin is not soluble in water, so we try to re-suspend it in NaOH 1M with work concentration of 50 μg/ml. Since bacterial cells still grow on our plates, we try to change the antibiotic.
So, we use a water-soluble Ampicillin with work concentration of 100 μg/ml. We find that it finally worked.
- We also have some problems with Kanamycin. We first use it at work concentration of 20 μg/ml, but it doesn’t work. Guys from the Boston University team have been so kind to tell us to use Kanamycin at a work concentration of 50 μg/ml and it works correctly.
- Preparation of 100 mM of IPTG stocks.
