NYMU Taipei/Lab Notes/2007 8 29

From 2007.igem.org

(Difference between revisions)
Line 9: Line 9:
** INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse) for insulin project
** INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse) for insulin project
** PUM1, AKAP1 for m.t. project
** PUM1, AKAP1 for m.t. project
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by 立翔

Revision as of 07:03, 30 August 2007

  • Plasmid extraction:
    • pOmpC+EYFP 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
    • Extract from 1.5ml LB medium to 20ul EB buffer
    • Stored in -20°C
  • EYFP spectral characteristics:
    • Excitation: 514nm
    • Emition: 527nm
  • Liquid culture for MGC clones
    • INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse) for insulin project
    • PUM1, AKAP1 for m.t. project

by 立翔

Retrieved from "http://2007.igem.org/wiki/index.php/NYMU_Taipei/Lab_Notes/2007_8_29"