Week 8

From 2007.igem.org

(Difference between revisions)
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::'''08/21/07'''
::'''08/21/07'''
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*There aren't colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. We can't amplify this biobrick.
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*There aren't colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. We can't amplify these biobricks.
*[http://partsregistry.org/Part:BBa_J04631 J04631] digestion.  
*[http://partsregistry.org/Part:BBa_J04631 J04631] digestion.  
*Band extraction.  
*Band extraction.  

Revision as of 10:07, 3 September 2007

08/20/07
  • [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012] transformation.


08/21/07
  • There aren't colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. We can't amplify these biobricks.
  • [http://partsregistry.org/Part:BBa_J04631 J04631] digestion.
  • Band extraction.
  • [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])ligation.



08/22/07
  • There are no colonies for [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])


08/23/07
  • A new [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2 ul T4 Ligase).
  • [http://partsregistry.org/Part:BBa_R0051 R0051], [http://partsregistry.org/Part:BBa_I763012 I763012] digestion with Spe/Pst1.
  • [http://partsregistry.org/Part:BBa_P0412 P0412], [http://partsregistry.org/Part:BBa_J04631 J04631] digestion with Xba/Pst1.
  • Run and extraction from gel.
  • Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligations.



08/24/07
  • We plan new amplifications.


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