Paris/September 20
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| + | [[Paris/September 19|yesterday]] -- [[Paris/September 21|tomorrow]] <br> | ||
== DapA Wanner deletion: pKD13-DapA homologies PCR == | == DapA Wanner deletion: pKD13-DapA homologies PCR == | ||
Latest revision as of 18:06, 7 October 2007
DapA Wanner deletion: pKD13-DapA homologies PCR
The recombination have not worked with the overnight culture on the bench (see yesterday).
The new electroporation with the new cells has not worked .
The positive control of electroporation (pUC19) shows a few colonies (10) leading to the conclusion of electroporation problem. A new protocol of electrocompetent cells is needed.
