Mini and Midi prep

From 2007.igem.org

Latest revision as of 15:45, 20 September 2007

Equipment and Reagents to Be Supplied by User

When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. For more information, consult the appropriate material safety data sheets (MSDSs), available from the product supplier.

For all protocols:

■ Standard microbiological equipment for growing and harvesting bacteria (e.g., inoculating loop, culture tubes and flasks, 37°C shaking incubator, and centrifuge with rotor and tubes or bottles for harvesting cells)

■ QIArack or equivalent holder (see “Setup of QIAGEN-tips”, page 13)

■ Ice

■ Isopropanol

■ 70% ethanol

■ Plasmid resuspension buffer (e.g., TE buffer, pH 8.0, or Tris·Cl, pH 8.5)

For QIAGEN Plasmid Mini Kit protocol:

■ Microcentrifuge

■ 1.5 ml or 2 ml microcentrifuge tubes

For QIAGEN Plasmid Midi, Maxi, Mega, and Giga Kit protocols:

■ Centrifugation tubes or vessels with suitable capacity for the volumes specified in the appropriate protocol.

■ Refrigerated centrifuge capable of ≥20,000 x g with rotor for the appropriate centrifuge tubes or bottles

Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Mini Kit

Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Midi and Maxi Kits